LOCUS       sequence-119639-        1093 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Standard for cell-free expression benchmarking..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1093)
  AUTHORS   Gagoski D, Polinkovsky ME, Mureev S, Kunert A, Johnston W, Gambin Y,
            Alexandrov K
  TITLE     Performance benchmarking of four cell-free protein expression 
            systems.
  JOURNAL   Biotechnol Bioeng. 2015 Aug 24. doi: 10.1002/bit.25814.
  PUBMED    26301602
REFERENCE   2  (bases 1 to 1093)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Biotechnol 
            Bioeng. 2015 Aug 24. doi: 10.1002/bit.25814."
COMMENT     Sequence Label: pCellFree_G03 CYP2D6
FEATURES             Location/Qualifiers
     source          1..1093
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    173..197
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     regulatory      251..260
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 tgcagggtcc acgccgtacc caacacacgg acacatgcac gaacacgcat gcagagagat
       61 catacatcat ctatagagaa gtacacgtaa aagtaagact cgtcgtctgc cagctacaac
      121 cgagaaggcg acgtgaaagg aacaagaaag gaggaggagg gcggccgctt acaccacttt
      181 gtacaagaaa gttgggcggg gcacagcaca aagctcatag ggggatgggg tcaccaggaa
      241 agcaaagaca ccatggtggc tgggccgggg ctgtccagtg ggcaccgaga agctgaagtg
      301 ctgcagcagg gaggtgaaga agaggaagag ctccatgcgg gccaggggct ccccgaggca
      361 tgcacggcgg cctgctgaga aaggcaggaa ggcctccggc ttcacaaagt ggccctgggc
      421 atccaggaag tgttcggggt ggaagcggaa gggcttctcc cagacggcct catccttcag
      481 caccgatgac aggttggtga tgagtgtcgt tcccttaggg atgcggaagc cctgtacttc
      541 gatgtcacgg gatgtcatat gggtcacacc cagggggacg atgtccccaa agcgctgcac
      601 ctcatgaatc acggcagtgg tgtagggcat gtgagcctgg tcacccatct ctggtcgccg
      661 cacctgccct atcacgtcgt cgatctcctg ttggacacgg cgctgcacat ccggatgtag
      721 gatcatgagc aggaggcccc aggccagcgt ggtcgaggtg gtcaccatcc cggcagagaa
      781 caggtcagcc accactatgc acaggttctc atcattgaag ctgctctcag ggttcccctt
      841 ggccttctcc atctctgcca ggaaggcctc agtcaggtct cgggggggct gggctgggtc
      901 ccaggtcatc ctgtgctcag ttagcagctc atccagctgg gtcaggaaag ccttttggaa
      961 gcgtaggacc ttgccagcca gcgctgggat atgcaggagg acggggacag cattcagcac
     1021 ctcgcgcaga aagcccgact cctccttcag tccctcctga gctaggtcca gcagcctgag
     1081 gaacaaggtc gtc
//
