LOCUS       sequence-119644-        9324 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  a binary Agrobacterium vector carrying a modified HSVtk gene as a 
            negative selection marker against ectopic transformants..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9324)
  AUTHORS   Khang CH, Park SY, Lee YH, Kang S
  TITLE     A dual selection based, targeted gene replacement tool for 
            Magnaporthe grisea and Fusarium oxysporum.
  JOURNAL   Fungal Genet Biol. 2005 Jun;42(6):483-92. Epub 2005 Apr 25.
  PUBMED    15893252
REFERENCE   2  (bases 1 to 9324)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Fungal 
            Genet Biol."; date: "2005-06"; volume: "42(6)"; pages: "483-92. Epub
            2005 Apr 25"
COMMENT     Sequence Label: pGKO2
FEATURES             Location/Qualifiers
     source          1..9324
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    1..25
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     polyA_signal    103..277
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     primer_bind     494..516
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     508..525
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     509..525
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     535..554
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        535..553
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     578..594
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     579..595
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(629..645)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(629..645)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     CDS             1095..2225
                     /codon_start=1
                     /product="herpes simplex virus thymidine kinase"
                     /label=HSV TK
                     /note="confers sensitivity to nucleoside analogs such as 
                     acyclovir or ganciclovir"
                     /translation="MASYPCHQHASAFDQAARSRGHSNRRTALRPRRQQEATEVRLEQK
                     MPTLLRVYIDGPHGMGKTTTTQLLVALGSRDDIVYVPEPMTYWQVLGASETIANIYTTQ
                     HRLDQGEISAGDAAVVMTSAQITMGMPYAVTDAVLAPHIGGEAGSSHAPPPALTLIFDR
                     HPIAALLCYPAARYLMGSMTPQAVLAFVALIPPTLPGTNIVLGALPEDRHIDRLAKRQR
                     PGERLDLAMLAAIRRVYGLLANTVRYLQGGGSWREDWGQLSGTAVPPQGAEPQSNAGPR
                     PHIGDTLFTLFRAPELLAPNGDLYNVFAWALDVLAKRLRPMHVFILDYDQSPAGCRDAL
                     LQLTSGMVQTHVTTPGSIPTICDLARTFAREMGEAN"
     promoter        complement(2525..2543)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(2564..2580)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2564..2580)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(2577..2599)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    2588..2604
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(2612..2618,2619..2637,2638..2643))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2658..2679
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     misc_feature    3047..3071
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             4371..5000
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     CDS             5434..6501
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="GRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESWQA
                     AADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLSKR
                     DRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKPGR
                     VFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGEAL
                     ISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYRLA
                     RRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPILV
                     MRYRNLIEGEASAGS"
     rep_origin      6567..6761
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     primer_bind     complement(6997..7019)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     misc_feature    7105..7245
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     7160..7179
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     rep_origin      complement(7431..8019)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7511..7530)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(8106..8900)
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
ORIGIN
        1 tggcaggata tattgtggtg taaacaaatt gacgcttaga caacttaata acacattgcg
       61 gacgttttta atgtactgaa ttaacgccga attaattcgg gggatctgga ttttagtact
      121 ggattttggt tttaggaatt agaaatttta ttgatagaag tattttacaa atacaaatac
      181 atactaaggg tttcttatat gctcaacaca tgagcgaaac cctataggaa ccctaattcc
      241 cttatctggg aactactcac acattattat ggagaaactc gattggcaag ctgctctagc
      301 caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcaaa cattaacgct
      361 tacaatttcc attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc
      421 tcttcgctat tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta
      481 acgccagggt tttcccagtc acgacgttgt aaaacgacgg ccagtgagcg cgcgtaatac
      541 gactcactat agggcgaatt gggtaccggg ccccccctcg aggtcgacgg tatcgataag
      601 cttgatatcg aattcctgca gcccggggga tccactagtt ctagagcggc cgccaccgcg
      661 gtgaattcga attgggtact caaattggtt cccgcttgac gacattccga aacccccaat
      721 ttcgcggctt cgaatcgtgg ctacccttgg ccgaggttgc gatttctctg ccgtatctga
      781 caatgatcca accggccgga tgtgtgggag ggcgcaaagg gtgtaattgg gtctaggcgt
      841 gagctgcatg tccgatggca gaaaaagaac tactaaaact ctgtgacgtc ctcagggctg
      901 gtagctattt agcttgcccc tccctcaccc cacctagctc aaacccaaca aaacacagca
      961 ccgaccacaa aaatctgcct cttaccacct gctcatcacc ttttctcaca tataaagcag
     1021 tgagctcagc ccgatttcca ttcctcaatt caagtctatt aactctctca aagaggaacc
     1081 caatcttcag atccatggct tcgtacccct gccatcaaca cgcgtctgcg ttcgaccagg
     1141 ctgcgcgttc tcgcggccat agcaaccgac gtacggcgtt gcgccctcgc cggcagcaag
     1201 aagccacgga agtccgcctg gagcagaaaa tgcccacgct actgcgggtt tatatagacg
     1261 gtcctcacgg gatggggaaa accaccacca cgcaactgct ggtggccctg ggttcgcgcg
     1321 acgacatcgt ctacgtaccc gagccgatga cttactggca ggtgctgggg gcttccgaga
     1381 caatcgcgaa catctacacc acacaacacc gcctcgacca gggtgagatt tcggccgggg
     1441 acgcggcggt ggtaatgaca agcgcccaga taacaatggg catgccttat gccgtgaccg
     1501 acgccgttct ggctcctcat atcggggggg aggctggcag ctcacatgcc ccgcccccgg
     1561 ccctcaccct catcttcgac cgccatccca tcgccgccct cctgtgctac ccggccgcgc
     1621 gataccttat gggcagcatg accccccagg ccgtgctggc gttcgtggcc ctcatcccgc
     1681 cgaccttgcc cggcacaaac atcgtgttgg gggcccttcc ggaggacaga cacatcgacc
     1741 gcctggccaa acgccagcgc cccggcgagc ggcttgacct ggctatgctg gccgcgattc
     1801 gccgcgttta cgggctgctt gccaatacgg tgcggtatct ccagggcggc gggtcgtggc
     1861 gggaggattg gggacagctt tcggggacgg ccgtgccgcc ccagggtgcc gagccccaga
     1921 gcaacgcggg cccacgaccc catatcgggg acacgttatt taccctgttt cgggcccccg
     1981 agttgctggc ccccaacggc gacctgtaca acgtgtttgc ctgggccttg gacgtcttgg
     2041 ccaaacgcct ccgtcccatg cacgtcttta tcctggatta cgaccaatcg cccgccggct
     2101 gccgggacgc cctgctgcaa cttacctccg ggatggtcca gacccacgtc accacccccg
     2161 gctccatacc gacgatctgc gacctggcgc gcacgtttgc ccgcgagatg ggggaggcta
     2221 actgagtcga ggtgcaggca tgcatcattc cactcaacat tcaggctcct ctgcgcacgt
     2281 aaagtgccaa aggcaatacc ctgctcggtg gaatgccgcc gggcttgtcg attttacgca
     2341 catatgcgca ttcttgactt gaagcggagg agttcttcgt tgcgggttac agtgttttaa
     2401 taaaagaatg gtcaaatcaa actgctagat atacctgtca gacactctag ttgttgaccc
     2461 ctatactctt aatacatcag acagtacatg catgttgcat gatgataagc tccagctttt
     2521 gttcccttta gtgagggtta attccgagct tggcgtaatc atggtcatag ctgtttcctg
     2581 tgtgaaattg ttatccgctc acaattccac acaacatacg agccggaagh cataaagtgt
     2641 aaagcctggg gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc
     2701 gctttccagt cgggaaacct gtcgtgccag ctgcattaat gaatcggcca acgcgcgggg
     2761 agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg
     2821 gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca
     2881 gaatcagggg ataacgcagg aaagaacatg aaggctggcg taatagcgaa gaggcccgca
     2941 ccgatcgccc ttcccaacag ttgcgcagcc tgaatggcga atgctagagc agcttgagct
     3001 tggatcagat tgtcgtttcc cgccttcagt ttaaactatc agtgtttgac aggatatatt
     3061 ggcgggtaaa cctaagagaa aagagcgttt attagaataa cggatattta aaagggcgtg
     3121 aaaaggttta tccgttcgtc catttgtatg tgcatgccaa ccacagggtt cccctcggga
     3181 tcaaagtact ttgatccaac ccctccgctg ctatagtgca gtcggcttct gacgttcagt
     3241 gcagccgtct tctgaaaacg acatgtcgca caagtcctaa gttacgcgac aggctgccgc
     3301 cctgcccttt tcctggcgtt ttcttgtcgc gtgttttagt cgcataaagt agaatacttg
     3361 cgactagaac cggagacatt acgccatgaa caagagcgcc gccgctggcc tgctgggcta
     3421 tgcccgcgtc agcaccgacg accaggactt gaccaaccaa cgggccgaac tgcacgcggc
     3481 cggctgcacc aagctgtttt ccgagaagat caccggcacc aggcgcgacc gcccggagct
     3541 ggccaggatg cttgaccacc tacgccctgg cgacgttgtg acagtgacca ggctagaccg
     3601 cctggcccgc agcacccgcg acctactgga cattgccgag cgcatccagg aggccggcgc
     3661 gggcctgcgt agcctggcag agccgtgggc cgacaccacc acgccggccg gccgcatggt
     3721 gttgaccgtg ttcgccggca ttgccgagtt cgagcgttcc ctaatcatcg accgcacccg
     3781 gagcgggcgc gaggccgcca aggcccgagg cgtgaagttt ggcccccgcc ctaccctcac
     3841 cccggcacag atcgcgcacg cccgcgagct gatcgaccag gaaggccgca ccgtgaaaga
     3901 ggcggctgca ctgcttggcg tgcatcgctc gaccctgtac cgcgcacttg agcgcagcga
     3961 ggaagtgacg cccaccgagg ccaggcggcg cggtgccttc cgtgaggacg cattgaccga
     4021 ggccgacgcc ctggcggccg ccgagaatga acgccaagag gaacaagcat gaaaccgcac
     4081 caggacggcc aggacgaacc gtttttcatt accgaagaga tcgaggcgga gatgatcgcg
     4141 gccgggtacg tgttcgagcc gcccgcgcac gtctcaaccg tgcggctgca tgaaatcctg
     4201 gccggtttgt ctgatgccaa gctggcggcc tggccggcca gcttggccgc tgaagaaacc
     4261 gagcgccgcc gtctaaaaag gtgatgtgta tttgagtaaa acagcttgcg tcatgcggtc
     4321 gctgcgtata tgatgcgatg agtaaataaa caaatacgca aggggaacgc atgaaggtta
     4381 tcgctgtact taaccagaaa ggcgggtcag gcaagacgac catcgcaacc catctagccc
     4441 gcgccctgca actcgccggg gccgatgttc tgttagtcga ttccgatccc cagggcagtg
     4501 cccgcgattg ggcggccgtg cgggaagatc aaccgctaac cgttgtcggc atcgaccgcc
     4561 cgacgattga ccgcgacgtg aaggccatcg gccggcgcga cttcgtagtg atcgacggag
     4621 cgccccaggc ggcggacttg gctgtgtccg cgatcaaggc agccgacttc gtgctgattc
     4681 cggtgcagcc aagcccttac gacatatggg ccaccgccga cctggtggag ctggttaagc
     4741 agcgcattga ggtcacggat ggaaggctac aagcggcctt tgtcgtgtcg cgggcgatca
     4801 aaggcacgcg catcggcggt gaggttgccg aggcgctggc cgggtacgag ctgcccattc
     4861 ttgagtcccg tatcacgcag cgcgtgagct acccaggcac tgccgccgcc ggcacaaccg
     4921 ttcttgaatc agaacccgag ggcgacgctg cccgcgaggt ccaggcgctg gccgctgaaa
     4981 ttaaatcaaa actcatttga gttaatgagg taaagagaaa atgagcaaaa gcacaaacac
     5041 gctaagtgcc ggccgtccga gcgcacgcag cagcaaggct gcaacgttgg ccagcctggc
     5101 agacacgcca gccatgaagc gggtcaactt tcagttgccg gcggaggatc acaccaagct
     5161 gaagatgtac gcggtacgcc aaggcaagac cattaccgag ctgctatctg aatacatcgc
     5221 gcagctacca gagtaaatga gcaaatgaat aaatgagtag atgaatttta gcggctaaag
     5281 gaggcggcat ggaaaatcaa gaacaaccag gcaccgacgc cgtggaatgc cccatgtgtg
     5341 gaggaacggg cggttggcca ggcgtaagcg gctgggttgt ctgccggccc tgcaatggca
     5401 ctggaacccc caagcccgag gaatcggcgt gacggtcgca aaccatccgg cccggtacaa
     5461 atcggcgcgg cgctgggtga tgacctggtg gagaagttga aggccgcgca ggccgcccag
     5521 cggcaacgca tcgaggcaga agcacgcccc ggtgaatcgt ggcaagcggc cgctgatcga
     5581 atccgcaaag aatcccggca accgccggca gccggtgcgc cgtcgattag gaagccgccc
     5641 aagggcgacg agcaaccaga ttttttcgtt ccgatgctct atgacgtggg cacccgcgat
     5701 agtcgcagca tcatggacgt ggccgttttc cgtctgtcga agcgtgaccg acgagctggc
     5761 gaggtgatcc gctacgagct tccagacggg cacgtagagg tttccgcagg gccggccggc
     5821 atggccagtg tgtgggatta cgacctggta ctgatggcgg tttcccatct aaccgaatcc
     5881 atgaaccgat accgggaagg gaagggagac aagcccggcc gcgtgttccg tccacacgtt
     5941 gcggacgtac tcaagttctg ccggcgagcc gatggcggaa agcagaaaga cgacctggta
     6001 gaaacctgca ttcggttaaa caccacgcac gttgccatgc agcgtacgaa gaaggccaag
     6061 aacggccgcc tggtgacggt atccgagggt gaagccttga ttagccgcta caagatcgta
     6121 aagagcgaaa ccgggcggcc ggagtacatc gagatcgagc tagctgattg gatgtaccgc
     6181 gagatcacag aaggcaagaa cccggacgtg ctgacggttc accccgatta ctttttgatc
     6241 gatcccggca tcggccgttt tctctaccgc ctggcacgcc gcgccgcagg caaggcagaa
     6301 gccagatggt tgttcaagac gatctacgaa cgcagtggca gcgccggaga gttcaagaag
     6361 ttctgtttca ccgtgcgcaa gctgatcggg tcaaatgacc tgccggagta cgatttgaag
     6421 gaggaggcgg ggcaggctgg cccgatccta gtcatgcgct accgcaacct gatcgagggc
     6481 gaagcatccg ccggttccta atgtacggag cagatgctag ggcaaattgc cctagcaggg
     6541 gaaaaaggtc gaaaaggtct ctttcctgtg gatagcacgt acattgggaa cccaaagccg
     6601 tacattggga accggaaccc gtacattggg aacccaaagc cgtacattgg gaaccggtca
     6661 cacatgtaag tgactgatat aaaagagaaa aaaggcgatt tttccgccta aaactcttta
     6721 aaacttatta aaactcttaa aacccgcctg gcctgtgcat aactgtctgg ccagcgcaca
     6781 gccgaagagc tgcaaaaagc gcctaccctt cggtcgctgc gctccctacg ccccgccgct
     6841 tcgcgtcggc ctatcgcggc cgctggccgc tcaaaaatgg ctggcctacg gccaggcaat
     6901 ctaccagggc gcggacaagc cgcgccgtcg ccactcgacc gccggcgccc acatcaaggc
     6961 accctgcctc gcgcgtttcg gtgatgacgg tgaaaacctc tgacacatgc agctcccgga
     7021 gacggtcaca gcttgtctgt aagcggatgc cgggagcaga caagcccgtc agggcgcgtc
     7081 agcgggtgtt ggcgggtgtc ggggcgcagc catgacccag tcacgtagcg atagcggagt
     7141 gtatactggc ttaactatgc ggcatcagag cagattgtac tgagagtgca ccatatgcgg
     7201 tgtgaaatac cgcacagatg cgtaaggaga aaataccgca tcaggcgctc ttccgcttcc
     7261 tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc agctcactca
     7321 aaggcggtaa tacggttatc cacagaatca ggggataacg caggaaagaa catgtgagca
     7381 aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg
     7441 ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg
     7501 acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt
     7561 ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt
     7621 tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc
     7681 tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt
     7741 gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt
     7801 agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc
     7861 tacactagaa ggacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa
     7921 agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt
     7981 tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct
     8041 acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgcattct
     8101 aggtactaaa acaattcatc cagtaaaata taatatttta ttttctccca atcaggcttg
     8161 atccccagta agtcaaaaaa tagctcgaca tactgttctt ccccgatatc ctccctgatc
     8221 gaccggacgc agaaggcaat gtcataccac ttgtccgccc tgccgcttct cccaagatca
     8281 ataaagccac ttactttgcc atctttcaca aagatgttgc tgtctcccag gtcgccgtgg
     8341 gaaaagacaa gttcctcttc gggcttttcc gtctttaaaa aatcatacag ctcgcgcgga
     8401 tctttaaatg gagtgtcttc ttcccagttt tcgcaatcca catcggccag atcgttattc
     8461 agtaagtaat ccaattcggc taagcggctg tctaagctat tcgtataggg acaatccgat
     8521 atgtcgatgg agtgaaagag cctgatgcac tccgcataca gctcgataat cttttcaggg
     8581 ctttgttcat cttcatactc ttccgagcaa aggacgccat cggcctcact catgagcaga
     8641 ttgctccagc catcatgccg ttcaaagtgc aggacctttg gaacaggcag ctttccttcc
     8701 agccatagca tcatgtcctt ttcccgttcc acatcatagg tggtcccttt ataccggctg
     8761 tccgtcattt ttaaatatag gttttcattt tctcccacca gcttatatac cttagcagga
     8821 gacattcctt ccgtatcttt tacgcagcgg tatttttcga tcagtttttt caattccggt
     8881 gatattctca ttttagccat ttattatttc cttcctcttt tctacagtat ttaaagatac
     8941 cccaagaagc taattataac aagacgaact ccaattcact gttccttgca ttctaaaacc
     9001 ttaaatacca gaaaacagct ttttcaaagt tgttttcaaa gttggcgtat aacatagtat
     9061 cgacggagcc gattttgaaa ccgcggtgat cacaggcagc aacgctctgt catcgttaca
     9121 atcaacatgc taccctccgc gagatcatcc gtgtttcaaa cccggcagct tagttgccgt
     9181 tcttccgaat agcatcggta acatgagcaa agtctgccgc cttacaacgg ctctcccgct
     9241 gacgccgtcc cggactgatg ggctgcctgt atcgagtggt gattttgtgc cgagctgccg
     9301 gtcggggagc tgttggctgg ctgg
//
