LOCUS       sequence-119646-       11710 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  a binary Agrobacterium vector carrying a modified HSVtk gene as a 
            negative selection marker against ectopic transformants. Uses 
            Gateway cloning..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11710)
  AUTHORS   Khang CH, Park SY, Lee YH, Kang S
  TITLE     A dual selection based, targeted gene replacement tool for 
            Magnaporthe grisea and Fusarium oxysporum.
  JOURNAL   Fungal Genet Biol. 2005 Jun;42(6):483-92. Epub 2005 Apr 25.
  PUBMED    15893252
REFERENCE   2  (bases 1 to 11710)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Fungal 
            Genet Biol."; date: "2005-06"; volume: "42(6)"; pages: "483-92. Epub
            2005 Apr 25"
COMMENT     Sequence Label: pGKO2-Gateway
FEATURES             Location/Qualifiers
     source          1..11710
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    1..25
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     polyA_signal    103..277
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     primer_bind     494..516
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     508..525
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     509..525
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     535..554
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        535..553
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     578..594
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     579..595
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    614..845
                     /gene="mutant version of attP"
                     /label=attP2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     promoter        990..1092
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     CDS             1093..1752
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(1159..1178)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     CDS             2094..2399
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     2278..2297
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
     protein_bind    complement(2795..3026)
                     /gene="mutant version of attP"
                     /label=attP1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             3481..4611
                     /codon_start=1
                     /product="herpes simplex virus thymidine kinase"
                     /label=HSV TK
                     /note="confers sensitivity to nucleoside analogs such as 
                     acyclovir or ganciclovir"
                     /translation="MASYPCHQHASAFDQAARSRGHSNRRTALRPRRQQEATEVRLEQK
                     MPTLLRVYIDGPHGMGKTTTTQLLVALGSRDDIVYVPEPMTYWQVLGASETIANIYTTQ
                     HRLDQGEISAGDAAVVMTSAQITMGMPYAVTDAVLAPHIGGEAGSSHAPPPALTLIFDR
                     HPIAALLCYPAARYLMGSMTPQAVLAFVALIPPTLPGTNIVLGALPEDRHIDRLAKRQR
                     PGERLDLAMLAAIRRVYGLLANTVRYLQGGGSWREDWGQLSGTAVPPQGAEPQSNAGPR
                     PHIGDTLFTLFRAPELLAPNGDLYNVFAWALDVLAKRLRPMHVFILDYDQSPAGCRDAL
                     LQLTSGMVQTHVTTPGSIPTICDLARTFAREMGEAN"
     promoter        complement(4911..4929)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(4950..4966)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4950..4966)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(4963..4985)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    4974..4990
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(4998..5004,5005..5023,5024..5029))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5044..5065
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     misc_feature    5433..5457
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             6757..7386
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     CDS             7820..8887
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="GRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESWQA
                     AADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLSKR
                     DRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKPGR
                     VFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGEAL
                     ISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYRLA
                     RRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPILV
                     MRYRNLIEGEASAGS"
     rep_origin      8953..9147
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     primer_bind     complement(9383..9405)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     misc_feature    9491..9631
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     9546..9565
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     rep_origin      complement(9817..10405)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(9897..9916)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(10492..11286)
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
ORIGIN
        1 tggcaggata tattgtggtg taaacaaatt gacgcttaga caacttaata acacattgcg
       61 gacgttttta atgtactgaa ttaacgccga attaattcgg gggatctgga ttttagtact
      121 ggattttggt tttaggaatt agaaatttta ttgatagaag tattttacaa atacaaatac
      181 atactaaggg tttcttatat gctcaacaca tgagcgaaac cctataggaa ccctaattcc
      241 cttatctggg aactactcac acattattat ggagaaactc gattggcaag ctgctctagc
      301 caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcaaa cattaacgct
      361 tacaatttcc attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc
      421 tcttcgctat tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta
      481 acgccagggt tttcccagtc acgacgttgt aaaacgacgg ccagtgagcg cgcgtaatac
      541 gactcactat agggcgaatt gggtaccggg ccccccctcg aggtcgacgg tatcgataag
      601 cttgctggat ggcaaataat gattttattt tgactgatag tgacctgttc gttgcaacaa
      661 attgataagc aatgctttct tataatgcca actttgtaca agaaagctga acgagaaacg
      721 taaaatgata taaatatcaa tatattaaat tagattttgc ataaaaaaca gactacataa
      781 tactgtaaaa cacaacatat ccagtcacta tgaatcaact acttagatgg tattagtgac
      841 ctgtagtcga ctaagttggc agcatcaccc gacgcacttt gcgccgaata aatacctgtg
      901 acggaagatc acttcgcaga ataaataaat cctggtgtcc ctgttgatac cgggaagccc
      961 tgggccaact tttggcgaaa atgagacgtt gatcggcacg taagaggttc caactttcac
     1021 cataatgaaa taagatcact accgggcgta ttttttgagt tatcgagatt ttcaggagct
     1081 aaggaagcta aaatggagaa aaaaatcact ggatatacca ccgttgatat atcccaatgg
     1141 catcgtaaag aacattttga ggcatttcag tcagttgctc aatgtaccta taaccagacc
     1201 gttcagctgg atattacggc ctttttaaag accgtaaaga aaaataagca caagttttat
     1261 ccggccttta ttcacattct tgcccgcctg atgaatgctc atccggaatt ccgtatggca
     1321 atgaaagacg gtgagctggt gatatgggat agtgttcacc cttgttacac cgttttccat
     1381 gagcaaactg aaacgttttc atcgctctgg agtgaatacc acgacgattt ccggcagttt
     1441 ctacacatat attcgcaaga tgtggcgtgt tacggtgaaa acctggccta tttccctaaa
     1501 gggtttattg agaatatgtt tttcgtctca gccaatccct gggtgagttt caccagtttt
     1561 gatttaaacg tggccaatat ggacaacttc ttcgcccccg ttttcaccat gggcaaatat
     1621 tatacgcaag gcgacaaggt gctgatgccg ctggcgattc aggttcatca tgccgtctgt
     1681 gatggcttcc atgtcggcag aatgcttaat gaattacaac agtactgcga tgagtggcag
     1741 ggcggggcgt aatcgcgtgg atccggctta ctaaaagcca gataacagta tgcgtatttg
     1801 cgcgctgatt tttgcggtat aagaatatat actgatatgt atacccgaag tatgtcaaaa
     1861 agaggtgtgc tatgaagcag cgtattacag tgacagttga cagcgacagc tatcagttgc
     1921 tcaaggcata tatgatgtca atatctccgg tctggtaagc acaaccatgc agaatgaagc
     1981 ccgtcgtctg cgtgccgaac gctggaaagc ggaaaatcag gaagggatgg ctgaggtcgc
     2041 ccggtttatt gaaatgaacg gctcttttgc tgacgagaac agggactggt gaaatgcagt
     2101 ttaaggttta cacctataaa agagagagcc gttatcgtct gtttgtggat gtacagagtg
     2161 atattattga cacgcccggg cgacggatgg tgatccccct ggccagtgca cgtctgctgt
     2221 cagataaagt ctcccgtgaa ctttacccgg tggtgcatat cggggatgaa agctggcgca
     2281 tgatgaccac cgatatggcc agtgtgccgg tctccgttat cggggaagaa gtggctgatc
     2341 tcagccaccg cgaaaatgac atcaaaaacg ccattaacct gatgttctgg ggaatataaa
     2401 tgtcaggctc ccttatacac agccagtctg caggtcgata cagtagaaat tacagaaact
     2461 ttatcacgtt tagtaagtat agaggctgaa aatccagatg aagccgaacg acttgtaaga
     2521 gaaaagtata agagttgtga aattgttctt gatgcagatg attttcagga ctatgacact
     2581 agcgtatatg aataggtaga tgtttttatt ttgtcacaca aaaaagaggc tcgcacctct
     2641 ttttcttatt tctttttatg atttaatacg gcattgagga caatagcgag taggctggat
     2701 acgacgattc cgtttgagaa gaacatttgg aaggctgtcg gtcgactaag ttggcagcat
     2761 cacccgaaga acatttggaa ggctgtcggt cgactacagg tcactaatac catctaagta
     2821 gttgattcat agtgactgga tatgttgtgt tttacagtat tatgtagtct gttttttatg
     2881 caaaatctaa tttaatatat tgatatttat atcattttac gcttctcgtt cagctttttt
     2941 gtacaaagtt ggcattataa aaaagcattg ctcatcaatt tgttgcaacg aacaggtcac
     3001 tatcagtcaa ataaaatcat tattttctag agcggccgcc accgcggtga attcgaattg
     3061 ggtactcaaa ttggttcccg cttgacgaca ttccgaaacc cccaatttcg cggcttcgaa
     3121 tcgtggctac ccttggccga ggttgcgatt tctctgccgt atctgacaat gatccaaccg
     3181 gccggatgtg tgggagggcg caaagggtgt aattgggtct aggcgtgagc tgcatgtccg
     3241 atggcagaaa aagaactact aaaactctgt gacgtcctca gggctggtag ctatttagct
     3301 tgcccctccc tcaccccacc tagctcaaac ccaacaaaac acagcaccga ccacaaaaat
     3361 ctgcctctta ccacctgctc atcacctttt ctcacatata aagcagtgag ctcagcccga
     3421 tttccattcc tcaattcaag tctattaact ctctcaaaga ggaacccaat cttcagatcc
     3481 atggcttcgt acccctgcca tcaacacgcg tctgcgttcg accaggctgc gcgttctcgc
     3541 ggccatagca accgacgtac ggcgttgcgc cctcgccggc agcaagaagc cacggaagtc
     3601 cgcctggagc agaaaatgcc cacgctactg cgggtttata tagacggtcc tcacgggatg
     3661 gggaaaacca ccaccacgca actgctggtg gccctgggtt cgcgcgacga catcgtctac
     3721 gtacccgagc cgatgactta ctggcaggtg ctgggggctt ccgagacaat cgcgaacatc
     3781 tacaccacac aacaccgcct cgaccagggt gagatttcgg ccggggacgc ggcggtggta
     3841 atgacaagcg cccagataac aatgggcatg ccttatgccg tgaccgacgc cgttctggct
     3901 cctcatatcg ggggggaggc tggcagctca catgccccgc ccccggccct caccctcatc
     3961 ttcgaccgcc atcccatcgc cgccctcctg tgctacccgg ccgcgcgata ccttatgggc
     4021 agcatgaccc cccaggccgt gctggcgttc gtggccctca tcccgccgac cttgcccggc
     4081 acaaacatcg tgttgggggc ccttccggag gacagacaca tcgaccgcct ggccaaacgc
     4141 cagcgccccg gcgagcggct tgacctggct atgctggccg cgattcgccg cgtttacggg
     4201 ctgcttgcca atacggtgcg gtatctccag ggcggcgggt cgtggcggga ggattgggga
     4261 cagctttcgg ggacggccgt gccgccccag ggtgccgagc cccagagcaa cgcgggccca
     4321 cgaccccata tcggggacac gttatttacc ctgtttcggg cccccgagtt gctggccccc
     4381 aacggcgacc tgtacaacgt gtttgcctgg gccttggacg tcttggccaa acgcctccgt
     4441 cccatgcacg tctttatcct ggattacgac caatcgcccg ccggctgccg ggacgccctg
     4501 ctgcaactta cctccgggat ggtccagacc cacgtcacca cccccggctc cataccgacg
     4561 atctgcgacc tggcgcgcac gtttgcccgc gagatggggg aggctaactg agtcgaggtg
     4621 caggcatgca tcattccact caacattcag gctcctctgc gcacgtaaag tgccaaaggc
     4681 aataccctgc tcggtggaat gccgccgggc ttgtcgattt tacgcacata tgcgcattct
     4741 tgacttgaag cggaggagtt cttcgttgcg ggttacagtg ttttaataaa agaatggtca
     4801 aatcaaactg ctagatatac ctgtcagaca ctctagttgt tgacccctat actcttaata
     4861 catcagacag tacatgcatg ttgcatgatg ataagctcca gcttttgttc cctttagtga
     4921 gggttaattc cgagcttggc gtaatcatgg tcatagctgt ttcctgtgtg aaattgttat
     4981 ccgctcacaa ttccacacaa catacgagcc ggaaghcata aagtgtaaag cctggggtgc
     5041 ctaatgagtg agctaactca cattaattgc gttgcgctca ctgcccgctt tccagtcggg
     5101 aaacctgtcg tgccagctgc attaatgaat cggccaacgc gcggggagag gcggtttgcg
     5161 tattgggcgc tcttccgctt cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg
     5221 gcgagcggta tcagctcact caaaggcggt aatacggtta tccacagaat caggggataa
     5281 cgcaggaaag aacatgaagg ctggcgtaat agcgaagagg cccgcaccga tcgcccttcc
     5341 caacagttgc gcagcctgaa tggcgaatgc tagagcagct tgagcttgga tcagattgtc
     5401 gtttcccgcc ttcagtttaa actatcagtg tttgacagga tatattggcg ggtaaaccta
     5461 agagaaaaga gcgtttatta gaataacgga tatttaaaag ggcgtgaaaa ggtttatccg
     5521 ttcgtccatt tgtatgtgca tgccaaccac agggttcccc tcgggatcaa agtactttga
     5581 tccaacccct ccgctgctat agtgcagtcg gcttctgacg ttcagtgcag ccgtcttctg
     5641 aaaacgacat gtcgcacaag tcctaagtta cgcgacaggc tgccgccctg cccttttcct
     5701 ggcgttttct tgtcgcgtgt tttagtcgca taaagtagaa tacttgcgac tagaaccgga
     5761 gacattacgc catgaacaag agcgccgccg ctggcctgct gggctatgcc cgcgtcagca
     5821 ccgacgacca ggacttgacc aaccaacggg ccgaactgca cgcggccggc tgcaccaagc
     5881 tgttttccga gaagatcacc ggcaccaggc gcgaccgccc ggagctggcc aggatgcttg
     5941 accacctacg ccctggcgac gttgtgacag tgaccaggct agaccgcctg gcccgcagca
     6001 cccgcgacct actggacatt gccgagcgca tccaggaggc cggcgcgggc ctgcgtagcc
     6061 tggcagagcc gtgggccgac accaccacgc cggccggccg catggtgttg accgtgttcg
     6121 ccggcattgc cgagttcgag cgttccctaa tcatcgaccg cacccggagc gggcgcgagg
     6181 ccgccaaggc ccgaggcgtg aagtttggcc cccgccctac cctcaccccg gcacagatcg
     6241 cgcacgcccg cgagctgatc gaccaggaag gccgcaccgt gaaagaggcg gctgcactgc
     6301 ttggcgtgca tcgctcgacc ctgtaccgcg cacttgagcg cagcgaggaa gtgacgccca
     6361 ccgaggccag gcggcgcggt gccttccgtg aggacgcatt gaccgaggcc gacgccctgg
     6421 cggccgccga gaatgaacgc caagaggaac aagcatgaaa ccgcaccagg acggccagga
     6481 cgaaccgttt ttcattaccg aagagatcga ggcggagatg atcgcggccg ggtacgtgtt
     6541 cgagccgccc gcgcacgtct caaccgtgcg gctgcatgaa atcctggccg gtttgtctga
     6601 tgccaagctg gcggcctggc cggccagctt ggccgctgaa gaaaccgagc gccgccgtct
     6661 aaaaaggtga tgtgtatttg agtaaaacag cttgcgtcat gcggtcgctg cgtatatgat
     6721 gcgatgagta aataaacaaa tacgcaaggg gaacgcatga aggttatcgc tgtacttaac
     6781 cagaaaggcg ggtcaggcaa gacgaccatc gcaacccatc tagcccgcgc cctgcaactc
     6841 gccggggccg atgttctgtt agtcgattcc gatccccagg gcagtgcccg cgattgggcg
     6901 gccgtgcggg aagatcaacc gctaaccgtt gtcggcatcg accgcccgac gattgaccgc
     6961 gacgtgaagg ccatcggccg gcgcgacttc gtagtgatcg acggagcgcc ccaggcggcg
     7021 gacttggctg tgtccgcgat caaggcagcc gacttcgtgc tgattccggt gcagccaagc
     7081 ccttacgaca tatgggccac cgccgacctg gtggagctgg ttaagcagcg cattgaggtc
     7141 acggatggaa ggctacaagc ggcctttgtc gtgtcgcggg cgatcaaagg cacgcgcatc
     7201 ggcggtgagg ttgccgaggc gctggccggg tacgagctgc ccattcttga gtcccgtatc
     7261 acgcagcgcg tgagctaccc aggcactgcc gccgccggca caaccgttct tgaatcagaa
     7321 cccgagggcg acgctgcccg cgaggtccag gcgctggccg ctgaaattaa atcaaaactc
     7381 atttgagtta atgaggtaaa gagaaaatga gcaaaagcac aaacacgcta agtgccggcc
     7441 gtccgagcgc acgcagcagc aaggctgcaa cgttggccag cctggcagac acgccagcca
     7501 tgaagcgggt caactttcag ttgccggcgg aggatcacac caagctgaag atgtacgcgg
     7561 tacgccaagg caagaccatt accgagctgc tatctgaata catcgcgcag ctaccagagt
     7621 aaatgagcaa atgaataaat gagtagatga attttagcgg ctaaaggagg cggcatggaa
     7681 aatcaagaac aaccaggcac cgacgccgtg gaatgcccca tgtgtggagg aacgggcggt
     7741 tggccaggcg taagcggctg ggttgtctgc cggccctgca atggcactgg aacccccaag
     7801 cccgaggaat cggcgtgacg gtcgcaaacc atccggcccg gtacaaatcg gcgcggcgct
     7861 gggtgatgac ctggtggaga agttgaaggc cgcgcaggcc gcccagcggc aacgcatcga
     7921 ggcagaagca cgccccggtg aatcgtggca agcggccgct gatcgaatcc gcaaagaatc
     7981 ccggcaaccg ccggcagccg gtgcgccgtc gattaggaag ccgcccaagg gcgacgagca
     8041 accagatttt ttcgttccga tgctctatga cgtgggcacc cgcgatagtc gcagcatcat
     8101 ggacgtggcc gttttccgtc tgtcgaagcg tgaccgacga gctggcgagg tgatccgcta
     8161 cgagcttcca gacgggcacg tagaggtttc cgcagggccg gccggcatgg ccagtgtgtg
     8221 ggattacgac ctggtactga tggcggtttc ccatctaacc gaatccatga accgataccg
     8281 ggaagggaag ggagacaagc ccggccgcgt gttccgtcca cacgttgcgg acgtactcaa
     8341 gttctgccgg cgagccgatg gcggaaagca gaaagacgac ctggtagaaa cctgcattcg
     8401 gttaaacacc acgcacgttg ccatgcagcg tacgaagaag gccaagaacg gccgcctggt
     8461 gacggtatcc gagggtgaag ccttgattag ccgctacaag atcgtaaaga gcgaaaccgg
     8521 gcggccggag tacatcgaga tcgagctagc tgattggatg taccgcgaga tcacagaagg
     8581 caagaacccg gacgtgctga cggttcaccc cgattacttt ttgatcgatc ccggcatcgg
     8641 ccgttttctc taccgcctgg cacgccgcgc cgcaggcaag gcagaagcca gatggttgtt
     8701 caagacgatc tacgaacgca gtggcagcgc cggagagttc aagaagttct gtttcaccgt
     8761 gcgcaagctg atcgggtcaa atgacctgcc ggagtacgat ttgaaggagg aggcggggca
     8821 ggctggcccg atcctagtca tgcgctaccg caacctgatc gagggcgaag catccgccgg
     8881 ttcctaatgt acggagcaga tgctagggca aattgcccta gcaggggaaa aaggtcgaaa
     8941 aggtctcttt cctgtggata gcacgtacat tgggaaccca aagccgtaca ttgggaaccg
     9001 gaacccgtac attgggaacc caaagccgta cattgggaac cggtcacaca tgtaagtgac
     9061 tgatataaaa gagaaaaaag gcgatttttc cgcctaaaac tctttaaaac ttattaaaac
     9121 tcttaaaacc cgcctggcct gtgcataact gtctggccag cgcacagccg aagagctgca
     9181 aaaagcgcct acccttcggt cgctgcgctc cctacgcccc gccgcttcgc gtcggcctat
     9241 cgcggccgct ggccgctcaa aaatggctgg cctacggcca ggcaatctac cagggcgcgg
     9301 acaagccgcg ccgtcgccac tcgaccgccg gcgcccacat caaggcaccc tgcctcgcgc
     9361 gtttcggtga tgacggtgaa aacctctgac acatgcagct cccggagacg gtcacagctt
     9421 gtctgtaagc ggatgccggg agcagacaag cccgtcaggg cgcgtcagcg ggtgttggcg
     9481 ggtgtcgggg cgcagccatg acccagtcac gtagcgatag cggagtgtat actggcttaa
     9541 ctatgcggca tcagagcaga ttgtactgag agtgcaccat atgcggtgtg aaataccgca
     9601 cagatgcgta aggagaaaat accgcatcag gcgctcttcc gcttcctcgc tcactgactc
     9661 gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg
     9721 gttatccaca gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa
     9781 ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga
     9841 cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag
     9901 ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct
     9961 taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg
    10021 ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc
    10081 ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt
    10141 aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta
    10201 tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaaggac
    10261 agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc
    10321 ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat
    10381 tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc
    10441 tcagtggaac gaaaactcac gttaagggat tttggtcatg cattctaggt actaaaacaa
    10501 ttcatccagt aaaatataat attttatttt ctcccaatca ggcttgatcc ccagtaagtc
    10561 aaaaaatagc tcgacatact gttcttcccc gatatcctcc ctgatcgacc ggacgcagaa
    10621 ggcaatgtca taccacttgt ccgccctgcc gcttctccca agatcaataa agccacttac
    10681 tttgccatct ttcacaaaga tgttgctgtc tcccaggtcg ccgtgggaaa agacaagttc
    10741 ctcttcgggc ttttccgtct ttaaaaaatc atacagctcg cgcggatctt taaatggagt
    10801 gtcttcttcc cagttttcgc aatccacatc ggccagatcg ttattcagta agtaatccaa
    10861 ttcggctaag cggctgtcta agctattcgt atagggacaa tccgatatgt cgatggagtg
    10921 aaagagcctg atgcactccg catacagctc gataatcttt tcagggcttt gttcatcttc
    10981 atactcttcc gagcaaagga cgccatcggc ctcactcatg agcagattgc tccagccatc
    11041 atgccgttca aagtgcagga cctttggaac aggcagcttt ccttccagcc atagcatcat
    11101 gtccttttcc cgttccacat cataggtggt ccctttatac cggctgtccg tcatttttaa
    11161 atataggttt tcattttctc ccaccagctt atatacctta gcaggagaca ttccttccgt
    11221 atcttttacg cagcggtatt tttcgatcag ttttttcaat tccggtgata ttctcatttt
    11281 agccatttat tatttccttc ctcttttcta cagtatttaa agatacccca agaagctaat
    11341 tataacaaga cgaactccaa ttcactgttc cttgcattct aaaaccttaa ataccagaaa
    11401 acagcttttt caaagttgtt ttcaaagttg gcgtataaca tagtatcgac ggagccgatt
    11461 ttgaaaccgc ggtgatcaca ggcagcaacg ctctgtcatc gttacaatca acatgctacc
    11521 ctccgcgaga tcatccgtgt ttcaaacccg gcagcttagt tgccgttctt ccgaatagca
    11581 tcggtaacat gagcaaagtc tgccgcctta caacggctct cccgctgacg ccgtcccgga
    11641 ctgatgggct gcctgtatcg agtggtgatt ttgtgccgag ctgccggtcg gggagctgtt
    11701 ggctggctgg
//
