LOCUS       sequence-119647-         982 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Targets HA-eGFP to mitochondrial intermembrane space (IMS).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 982)
  AUTHORS   Kerr SC, Kahn RA
  TITLE     Tool box: Plasmids for the expression or knockdown of human ARF 
            Family GTPases (ARF/ARL/SAR) and their co-expression in bacteria 
            with N-myristoyltransferases.
  JOURNAL   Cell Logist. 2015 Sep 21;5(3):e1090523. doi: 
            10.1080/21592799.2015.1090523. eCollection 2015 Jul-Sep.
  PUBMED    27057421
REFERENCE   2  (bases 1 to 982)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell 
            Logist."; date: "2015-09-21"; volume: "5(3):e1090523. doi"; pages: "
            10.1080/21592799.2015.1090523. eCollection 2015 Jul-Sep"
COMMENT     Sequence Label: pCMV2-SMAC-HA-eGFP
FEATURES             Location/Qualifiers
     source          1..982
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(18..44)
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     regulatory      220..229
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(286..305)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(287..305)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        complement(350..449)
                     /label=CMVd1 promoter
                     /note="truncated human cytomegalovirus (CMV) immediate 
                     early promoter that yields slightly reduced expression 
                     relative to the full-length promoter"
     primer_bind     complement(379..399)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     688..707
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     promoter        782..886
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 cccttgctca cgaattcagc gtaatctgga acatcgtatg ggtaggatcc aataggaacc
       61 gcacacaggg ttactccaaa gccaatcgtc acagttttgt gccatggtct tatcaattct
      121 gagaaacacc gcttcttaaa gttagccaca acaggaacac acaaacactg tctgtacctg
      181 aagaatgaag ttacgctgcg cgacagccaa ctcttcagag ccgccatggt acctccggat
      241 cgataattct agagggcccg tttaaacgct agccagcttg ggtctcccta tagtgagtcg
      301 tattaatttc gataagccag taagcagtgg gttctctagt tagccagaga gctctgctta
      361 tatagacctc ccaccgtaca cgcctaccgc ccatttgcgt caatggggcg gagttgttac
      421 gacattttgg aaagtcccgt tgattttggt gccaaaacaa actcccattg acgtcaatgg
      481 ggtggagact tggaaatccc cgtgagtcaa accgctaacg cgtatatctg gcccgtacat
      541 cgcgaagcag cgcaaaacgc ctaaccctaa gcagattctt catgcaattg tcggtcaagc
      601 cttgccttgt tgtagcttaa attttgctcg cgcactactc agcgacctcc aacacacaag
      661 cagggagcag atactggctt aactatgcgg catcagagca gattgtactg agagtgcacc
      721 ataggggatc gggagatctc ccgatccgtc gacgtcaggt ggcacttttc ggggaaatgt
      781 gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag
      841 acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtatga gtattcaaca
      901 tttccgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt ttgctcaccc
      961 agaaacgctg gtgaaagtaa aa
//
