LOCUS       sequence-119649-         946 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Targets HA-eGFP to mitochondrial intermembrane space (IMS).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 946)
  AUTHORS   Kerr SC, Kahn RA
  TITLE     Tool box: Plasmids for the expression or knockdown of human ARF 
            Family GTPases (ARF/ARL/SAR) and their co-expression in bacteria 
            with N-myristoyltransferases.
  JOURNAL   Cell Logist. 2015 Sep 21;5(3):e1090523. doi: 
            10.1080/21592799.2015.1090523. eCollection 2015 Jul-Sep.
  PUBMED    27057421
REFERENCE   2  (bases 1 to 946)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell 
            Logist."; date: "2015-09-21"; volume: "5(3):e1090523. doi"; pages: "
            10.1080/21592799.2015.1090523. eCollection 2015 Jul-Sep"
COMMENT     Sequence Label: pCMV3-SMAC-HA-eGFP
FEATURES             Location/Qualifiers
     source          1..946
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(22..48)
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     regulatory      224..233
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(290..309)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(291..309)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        complement(354..453)
                     /label=CMVd1 promoter
                     /note="truncated human cytomegalovirus (CMV) immediate 
                     early promoter that yields slightly reduced expression 
                     relative to the full-length promoter"
     primer_bind     complement(383..403)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     658..677
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     promoter        752..856
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 ctcgcccttg ctcacgaatt cagcgtaatc tggaacatcg tatgggtagg atccaatagg
       61 aaccgcacac agggttactc caaagccaat cgtcacagtt ttgtgccatg gtcttatcaa
      121 ttctgagaaa caccgcttct taaagttagc cacaacagga acacacaaac actgtctgta
      181 cctgaagaat gaagttacgc tgcgcgacag ccaactcttc agagccgcca tggtacctcc
      241 ggatcgataa ttctagaggg cccgtttaaa cgctagccag cttgggtctc cctatagtga
      301 gtcgtattaa tttcgataag ccagtaagca gtgggttctc tagttagcca gagagctctg
      361 cttatataga cctcccaccg tacacgccta ccgcccattt gcgtcaatgg ggcggagttg
      421 ttacgacatt ttggaaagtc ccgttgattt tggtgccaaa acaaactccc attgacgtca
      481 atggggtacg cgtatatctg gcccgtacat cgcgaagcag cgcaaaacgc ctaaccctaa
      541 gcagattctt catgcaattg tcggtcaagc cttgccttgt tgtagcttaa attttgctcg
      601 cgcactactc agcgacctcc aacacacaag cagggagcag atactggctt aactatgcgg
      661 catcagagca gattgtactg agagtgcacc ataggggatc gggagatctc ccgatccgtc
      721 gacgtcaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta
      781 aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata
      841 ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc ccttttttgc
      901 ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaa
//
