LOCUS       sequence-119650-         980 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Targets HA-eGFP to mitochondrial intermembrane space (IMS).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 980)
  AUTHORS   Kerr SC, Kahn RA
  TITLE     Tool box: Plasmids for the expression or knockdown of human ARF 
            Family GTPases (ARF/ARL/SAR) and their co-expression in bacteria 
            with N-myristoyltransferases.
  JOURNAL   Cell Logist. 2015 Sep 21;5(3):e1090523. doi: 
            10.1080/21592799.2015.1090523. eCollection 2015 Jul-Sep.
  PUBMED    27057421
REFERENCE   2  (bases 1 to 980)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell 
            Logist."; date: "2015-09-21"; volume: "5(3):e1090523. doi"; pages: "
            10.1080/21592799.2015.1090523. eCollection 2015 Jul-Sep"
COMMENT     Sequence Label: pCMV4-SMAC-HA-eGFP
FEATURES             Location/Qualifiers
     source          1..980
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(22..48)
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     regulatory      224..233
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(290..309)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(291..309)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        complement(354..405)
                     /label=CMVd2 promoter
                     /note="truncated human cytomegalovirus (CMV) immediate 
                     early promoter that yields moderately reduced expression 
                     relative to the full-length promoter"
     primer_bind     complement(383..403)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     619..638
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     promoter        713..817
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 ctcgcccttg ctcacgaatt cagcgtaatc tggaacatcg tatgggtagg atccaatagg
       61 aaccgcacac agggttactc caaagccaat cgtcacagtt ttgtgccatg gtcttatcaa
      121 ttctgagaaa caccgcttct taaagttagc cacaacagga acacacaaac actgtctgta
      181 cctgaagaat gaagttacgc tgcgcgacag ccaactcttc agagccgcca tggtacctcc
      241 ggatcgataa ttctagaggg cccgtttaaa cgctagccag cttgggtctc cctatagtga
      301 gtcgtattaa tttcgataag ccagtaagca gtgggttctc tagttagcca gagagctctg
      361 cttatataga cctcccaccg tacacgccta ccgcccattt gcgtcaatgg ggcggagttg
      421 ttacgacatt ttggaaagtc ccgttgatac gcgtatatct ggcccgtaca tcgcgaagca
      481 gcgcaaaacg cctaacccta agcagattct tcatgcaatt gtcggtcaag ccttgccttg
      541 ttgtagctta aattttgctc gcgcactact cagcgacctc caacacacaa gcagggagca
      601 gatactggct taactatgcg gcatcagagc agattgtact gagagtgcac cataggggat
      661 cgggagatct cccgatccgt cgacgtcagg tggcactttt cggggaaatg tgcgcggaac
      721 ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc
      781 ctgataaatg cttcaataat attgaaaaag gaagagtatg agtattcaac atttccgtgt
      841 cgcccttatt cccttttttg cggcattttg ccttcctgtt tttgctcacc cagaaacgct
      901 ggtgaaagta aaagatgctg aagatcagtt gggtgcacga gtgggttaca tcgaactgga
      961 tctcaacagc ggtaagatcc
//
