LOCUS       sequence-119653-         391 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Tet-inducible expression of IDH2 in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 391)
  AUTHORS   Lewis CA, Parker SJ, Fiske BP, McCloskey D, Gui DY, Green CR, Vokes 
            NI, Feist AM, Vander Heiden MG, Metallo CM
  TITLE     Tracing compartmentalized NADPH metabolism in the cytosol and 
            mitochondria of mammalian cells.
  JOURNAL   Mol Cell. 2014 Jul 17;55(2):253-63. doi: 
            10.1016/j.molcel.2014.05.008. Epub 2014 May 29.
  PUBMED    24882210
REFERENCE   2  (bases 1 to 391)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.molcel.2014.05.008"; journalName: "Mol Cell"; date: 
            "2014-07-17- 17"; volume: "55"; issue: "2"; pages: "253-63"
COMMENT     Sequence Label: pSLIK-IDH2-FLAG
FEATURES             Location/Qualifiers
     source          1..391
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    152..176
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             complement(197..262)
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
ORIGIN
        1 gggcggagga caggagctcg ctgcgccctt cgtctgacgt ggcagcgctc gccgtgagga
       61 ggggggcgcc cgcgggaggc gccaaaaccc ggcgcggagg ccagatcttg ggtgggttaa
      121 ttaaccatct ttatggctcg agatgttatc aaccactttg tacaagaaag ctgggtctag
      181 atatctcgag tcactacttg tcatcgtcat ccttgtagtc gatgtcatga tctttataat
      241 caccgtcatg gtctttgtag tcagcccggg atcctctaga atactgcctg cccagggctc
      301 tgtccaggtt gctcttgatg gtgtcgagga agtccgtggt gttcaggaag tgctcgttca
      361 gcttcacatt gctgaggccg tgaatgcagc c
//
