LOCUS       sequence-119657-         818 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Targeting vector for PITT landing pad knock-in into the Rosa26 
            locus.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 818)
  AUTHORS   Ohtsuka M, Miura H, Mochida K, Hirose M, Hasegawa A, Ogura A, 
            Mizutani R, Kimura M, Isotani A, Ikawa M, Sato M, Gurumurthy CB
  TITLE     One-step generation of multiple transgenic mouse lines using an 
            improved Pronuclear Injection-based Targeted Transgenesis (i-PITT).
  JOURNAL   BMC Genomics. 2015 Apr 9;16:274. doi: 10.1186/s12864-015-1432-5.
  PUBMED    25887549
REFERENCE   2  (bases 1 to 818)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "BMC 
            Genomics."; date: "2015-04-9"; volume: "16:274. doi"; pages: " 
            10.1186/s12864-015-1432-5"
COMMENT     Sequence Label: pBDT
FEATURES             Location/Qualifiers
     source          1..818
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     polyA_signal    243..324
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(280..299)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     protein_bind    complement(325..372)
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     protein_bind    complement(373..406)
                     /label=lox2272
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (AAGTATCC) (Shaw et al., 2021). lox2272 sites are 
                     compatible with each other, but incompatible with loxP or 
                     loxN sites (Lee and Saito, 1988)."
ORIGIN
        1 cgcttcctcg tgctttacgg tatcgccgct cccgattcgc agcgcatcgc cttctatcgc
       61 cttcttgacg agttcttctg acccagacct ggcacccagg agaggagcag gcagggtcag
      121 ctgcgccgcg actctagatc ataatcagcc ataccacatt tgtagaggtt ttacttgctt
      181 taaaaaacct cccacacctc cccctgaacc tgaaacataa aatgaatgca attgttgttg
      241 ttaacttgtt tattgcagct tataatggtt acaaataaag caatagcatc acaaatttca
      301 caaataaagc atttttttca ctgcgaagtt cctatacttt ctagagaata tgaacttcgg
      361 aataggaact tcataacttc gtataggata ctttatacga agttatgaag ttcctatact
      421 cctataagaa taggaacttc ggaataggaa cttctaacta taacggtcct aaggtagcga
      481 tatcttctgg gcaggcttaa aggctaacct ggtgtgtggg cgttgtcctg caggggaatt
      541 gaacaggtgt aaaattggag ggacaagact tcccacagat tttcggtttt gtcgggaagt
      601 tttttaatag gggcaaataa ggaaaatggg aggataggta gtcatctggg gttttatgca
      661 gcaaaactac aggttattat tgcttgtgat ccgcctcgga gtattttcca tcgaggtaga
      721 ttaaagacat gctcacccga gttttatact ctcctgcttg agatccttac tacagtatga
      781 aattacagtg tcgcgagtta gactatgtaa gcagaatt
//
