LOCUS       sequence-119658-         777 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Targeting vector for PITT landing pad knock-in into the Rosa26 
            locus.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 777)
  AUTHORS   Ohtsuka M, Miura H, Mochida K, Hirose M, Hasegawa A, Ogura A, 
            Mizutani R, Kimura M, Isotani A, Ikawa M, Sato M, Gurumurthy CB
  TITLE     One-step generation of multiple transgenic mouse lines using an 
            improved Pronuclear Injection-based Targeted Transgenesis (i-PITT).
  JOURNAL   BMC Genomics. 2015 Apr 9;16:274. doi: 10.1186/s12864-015-1432-5.
  PUBMED    25887549
REFERENCE   2  (bases 1 to 777)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "BMC 
            Genomics."; date: "2015-04-9"; volume: "16:274. doi"; pages: " 
            10.1186/s12864-015-1432-5"
COMMENT     Sequence Label: pBDU
FEATURES             Location/Qualifiers
     source          1..777
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_feature    join(178..243,244..281)
                     /label=Ad SA
                     /note="adenovirus major late transcript splice acceptor 
                     (Friedrich and Soriano, 1991)"
     regulatory      445..454
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(496..517)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(757..776)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
ORIGIN
        1 gcggggagaa ggccgcaccc ttctccggag gggggagggg agtgttgcaa tacctttctg
       61 ggagttctct gctgcctcct ggcttctgag gaccgccctg ggcctgggag aatcccttcc
      121 ccctcttccc tcgtgatctg caactccagt ctgatatcaa aactctctta aggtagctag
      181 ggcgcagtag tccagggttt ccttgatgat gtcatactta tcctgtccct tttttttcca
      241 cagctcgcgg ttgaggacaa actcttcgcg gtctttccag tggggatcga cggtatcgat
      301 aagctgaagt tcctattccg aagttcctat tctatcagaa gtataggaac ttcgtagtgc
      361 cccaactggg gtaacctttg agttctctca gttgggggcg tagaattatt cgtatagcat
      421 acattatacg aagttatacc ggtcgccacc atggtgagca agggcgagga gctgttcacc
      481 ggggtggtgc ccatcctggt cgagctggac ggcgacgtaa acggccacaa gttcagcgtg
      541 tccggcgagg gcgagggcga tgccacctac ggcaagctga ccctgaagtt catctgcacc
      601 accggcaagc tgcccgtgcc ctggcccacc ctcgtgacca ccctgaccta cggcgtgcag
      661 tgcttcagcc gctaccccga ccacatgaag cagcacgact tcttcaagtc cgccatgccc
      721 gaaggctacg tccaggagcg caccatcttc ttcaaggacg acggcaacta caagacc
//
