LOCUS       sequence-119663-         853 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 853)
  TITLE     Other Plasmids
REFERENCE   2  (bases 1 to 853)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: V22 CS2+ RFPce
FEATURES             Location/Qualifiers
     source          1..853
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(join(47..715,716..718,719..721))
                     /codon_start=1
                     /product="wild-type DsRed"
                     /label=DsRed1
                     /note="mammalian codon-optimized"
                     /translation="MVRSSKNVIKEFMRFKVRMEGTVNGHEFEIEGEGEGRPYEGHNTV
                     KLKVTKGGPLPFAWDILSPQFQYGSKVYVKHPADIPDYKKLSFPEGFKWERVMNFEDGG
                     VVTVTQDSSLQDGCFIYKVKFIGVNFPSDGPVMQKKTMGWEASTERLYPRDGVLKGEIH
                     KALKLKDGGHYLVEFKSIYMAKKPVQLPGYYYVDSKLDITSHNEDYTIVEQYERTEGRH
                     HLF"
     primer_bind     complement(103..126)
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
     primer_bind     complement(301..320)
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     518..538
                     /label=DsRed1-N
                     /note="DsRed1, reverse primer"
     promoter        complement(762..780)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(763..780)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
ORIGIN
        1 cgactcacta tagttctaga ggctcgagag gccttgaatt cgaatcgaac aggtggtggc
       61 ggccctcggt gcgctcgtac tgctccacga tggtgtagtc ctcgttgtgg gaggtgatgt
      121 ccagcttgga gtccacgtag tagtagccgg gcagctgcac gggcttcttg gccatgtaga
      181 tggacttgaa ctccaccagg tagtggccgc cgtccttcag cttcagggcc ttgtggatct
      241 cgcccttcag cacgccgtcg cgggggtaca ggcgctcggt ggaggcctcc cagcccatgg
      301 tcttcttctg cattacgggg ccgtcggagg ggaagttcac gccgatgaac ttcaccttgt
      361 agatgaagca gccgtcctgc agggaggagt cctgggtcac ggtcaccacg ccgccgtcct
      421 cgaagttcat cacgcgctcc cacttgaagc cctcggggaa ggacagcttc ttgtagtcgg
      481 ggatgtcggc ggggtgcttc acgtacacct tggagccgta ctggaactgg ggggacagga
      541 tgtcccaggc gaagggcagg gggccgccct tggtcacctt cagcttcacg gtgttgtggc
      601 cctcgtaggg gcggccctcg ccctcgccct cgatctcgaa ctcgtggccg ttcacggtgc
      661 cctccatgcg caccttgaag cgcatgaact ccttgatgac gttcttggag gagcgcacca
      721 tcaatcgatg ggatcctgca aaaagaacaa gtagcttgta ttctatagtg tcacctaaat
      781 caagcttgct ccgacgtccc caggcagaat ggcggttccc taaacgagca ttgcttatat
      841 agacctccca tta
//
