LOCUS       sequence-119669-        1059 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Standard for cell-free expression benchmarking..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1059)
  AUTHORS   Gagoski D, Polinkovsky ME, Mureev S, Kunert A, Johnston W, Gambin Y,
            Alexandrov K
  TITLE     Performance benchmarking of four cell-free protein expression 
            systems.
  JOURNAL   Biotechnol Bioeng. 2015 Aug 24. doi: 10.1002/bit.25814.
  PUBMED    26301602
REFERENCE   2  (bases 1 to 1059)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Biotechnol 
            Bioeng. 2015 Aug 24. doi: 10.1002/bit.25814."
COMMENT     Sequence Label: pCellFree_G03 TFAP2B
FEATURES             Location/Qualifiers
     source          1..1059
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    173..197
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 tgcagggtac cacgcgtacc caacacacgg acacatgcac gaacacgcat gcagagagat
       61 catacatcat ctatagagaa gtacacgtaa aagtaagact cgtcgtctgc cagctacaac
      121 cgagaaggcg acgtgaaagg aacaagaaag gaggaggagg gcggccgctt acaccacttt
      181 gtacaagaaa gctgggtcca atttcctgtg tttctcctcc ttgtcgccag ttttactacc
      241 tgggccttcc ccagacgtgt gcctgttagt ggtggtgttg ttcaagaaca tcttgtccat
      301 gcctttgagc gcctcggtga gatagttctg cagggccgtg agcgcggcgc aaatggccgg
      361 ggcgccgaag ccgtgcgtga tgaggctgaa gtgcgtgagg cagctctgga tccccggctc
      421 caggatgggg ctgggtcggc tgttccctat cggtgtccgg tcctgcgcca gtagatccgt
      481 aaattcttta caaagttgct tggtggccaa cagcatattc tttcgggagt gcaggtcact
      541 cgggtctgtg tgctgccggt tcaaatactc agagacggct ttggcgggaa actccgtttc
      601 gcaaatgtac ccaaaatccc tagctaagtg aacagcttct ccttccacca gggaggtgag
      661 taacgtgaca tttgctgctt tgcgcctgcc cgcgggtaaa ttcaaaccga ttttttctag
      721 cctttctcgc aaagatctcc ccccattttt cgatttggct cttctgagga ctccgccgag
      781 gagagatgca ttgaggcatt cagggggcga cagccgtctc tgaacttctc ccacagttac
      841 tttgtacttc gaagttgaac tgagcagaga caaacggcct gggacggagc aaaacacctc
      901 gccggtgttg acagacatgc ctcccaggaa gccgtcttta ttcatcatta gagaagtcac
      961 cgatttggga ggaactggaa cttttttaat gacagactgg tccaatagat tcatgccgct
     1021 gttattggca tcttcaactg actggacgtc ttccattcc
//
