LOCUS       sequence-119670-         880 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses UBE2S (1-196) in E. coli.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 880)
  AUTHORS   Bremm A, Freund SM, Komander D
  TITLE     Lys11-linked ubiquitin chains adopt compact conformations and are 
            preferentially hydrolyzed by the deubiquitinase Cezanne.
  JOURNAL   Nat Struct Mol Biol. 2010 Aug;17(8):939-47. doi: 10.1038/nsmb.1873. 
            Epub 2010 Jul 11.
  PUBMED    20622874
REFERENCE   2  (bases 1 to 880)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/nsmb.1873";
            journalName: "Nat Struct Mol Biol"; date: "2010-08"; volume: "17"; 
            issue: "8"; pages: "939-47"
COMMENT     Sequence Label: pOPINK-UBE2S
FEATURES             Location/Qualifiers
     source          1..880
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             629..646
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(699..717)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      713..760
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
ORIGIN
        1 ttctgtttca gggcccgatg aactccaacg tggagaacct acccccgcac atcatccgcc
       61 tggtgtacaa ggaggtgacg acactgaccg cagacccacc cgatggcatc aaggtctttc
      121 ccaacgagga ggacctcacc gacctccagg tcaccatcga gggccctgag gggaccccat
      181 atgctggagg tctgttccgc atgaaactcc tgctggggaa ggacttccct gcctccccac
      241 ccaagggcta cttcctgacc aagatcttcc acccgaacgt gggcgccaat ggcgagatct
      301 gcgtcaacgt gctcaagagg gactggacgg ctgagctggg catccgacac gtactgctga
      361 ccatcaagtg cctgctgatc caccctaacc ccgagtctgc actcaacgag gaggcgggcc
      421 gcctgctctt ggagaactac gaggagtatg cagctcgggc ccgtctgctc acagagatcc
      481 acgggggcgc cggcgggccc agcggcaggg ccgaagccgg tcgggccctg gccagtggca
      541 ctgaagcttc ctccaccgac cctggggccc cagggggccc gggaggggct gagggtccca
      601 tggcctaaag ctttctagac catttaaaca ccaccaccac caccactgag atccggctgc
      661 taacaaagcc cgaaaggaag ctgagttggc tgctgccacc gctgagcaat aactagcata
      721 accccttggg gcctctaaac gggtcttgag gggttttttg ctgaaaggag gaactatatc
      781 cggattggcg aatgggacgc gccctgtagc ggcgcattaa gcgcggcggg tgtggtggtt
      841 acgcgcagcg tgaccgctac acttgcccag cgccctagcg
//
