LOCUS       sequence-119676-        1034 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Standard for cell-free expression benchmarking..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1034)
  AUTHORS   Gagoski D, Polinkovsky ME, Mureev S, Kunert A, Johnston W, Gambin Y,
            Alexandrov K
  TITLE     Performance benchmarking of four cell-free protein expression 
            systems.
  JOURNAL   Biotechnol Bioeng. 2015 Aug 24. doi: 10.1002/bit.25814.
  PUBMED    26301602
REFERENCE   2  (bases 1 to 1034)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Biotechnol 
            Bioeng. 2015 Aug 24. doi: 10.1002/bit.25814."
COMMENT     Sequence Label: pCellFree_G03 GDI2
FEATURES             Location/Qualifiers
     source          1..1034
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    168..192
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 ggtaccacgc gtacccaaca cacggacaca tgcacgaaca cgcatgcaga gagatcatac
       61 atcatctata gagaagtaca cgtaaaagta agactcgtcg tctgccagct acaaccgaga
      121 aggcgacgtg aaaggaacaa gaaaggagga ggagggcggc cgcttacacc actttgtaca
      181 agaaagttgg gtagtctccc catagatgtc attcttcttg cgcttcattt cctcaaagtc
      241 aaactctgat cctgtcatcc tcttatagat gtttttaatg tcatcacacg ttgtctcaaa
      301 atgagtggtg gcatcatatg tgcgggaaat aaagatctgg ctttctgttc ccaagtcttt
      361 tggtaccagg aggtcactga tgctaacaaa tttctgttca attggttcca agagctccaa
      421 agctggtctg atttccttct caggctcctt ggtttccaca gttgtactaa ctatagcaat
      481 gtacttccct tgtgctgcta cattgtgcgc aaaggagatc atgcagacgt agatatctga
      541 ctttcgattg acttggttct gtggaataat gatctggcag gagttggcat cattggtgtt
      601 cttgatgggg tggctgagga tgcaaataac tctgatcacc tggcccactt tttctacccg
      661 atcttttacg tagctggggt cacagatgag ctgcttacag cgagcaattt ctccttcaga
      721 ttttacacca attacttttc cattctgtac aatgatttct tcaatgggtt tattcagcat
      781 ataggtacct ccataaatag cacttagcct tgcaaatcct tggggcagtt ctccaaggcc
      841 atagagtgga taaaggtatg ggcttttgcc atatcttgcc aaagattcac tgtaaagttt
      901 aattctatta atggtttcat aacacggttg atctaagtaa tcatcagttc tgtaaagtgc
      961 aagagcatga ccagtaaaat ctataacgtc ttgacccaaa tcaaatttct tatacacatc
     1021 tcgcattgtg gtct
//
