LOCUS       sequence-119680-        1056 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Standard for cell-free expression benchmarking..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1056)
  AUTHORS   Gagoski D, Polinkovsky ME, Mureev S, Kunert A, Johnston W, Gambin Y,
            Alexandrov K
  TITLE     Performance benchmarking of four cell-free protein expression 
            systems.
  JOURNAL   Biotechnol Bioeng. 2015 Aug 24. doi: 10.1002/bit.25814.
  PUBMED    26301602
REFERENCE   2  (bases 1 to 1056)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Biotechnol 
            Bioeng. 2015 Aug 24. doi: 10.1002/bit.25814."
COMMENT     Sequence Label: pCellFree_G03 GTF2H4
FEATURES             Location/Qualifiers
     source          1..1056
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(21..44)
                     /codon_start=1
                     /product="8xHis affinity tag"
                     /label=8xHis
                     /translation="HHHHHHHH"
     primer_bind     complement(182..201)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(183..201)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        233..337
                     /gene="bla"
                     /label=AmpR promoter
     CDS             522..900
                     /label=ISS
                     /note="immunostimulatory sequence from the AmpR gene; 
                     contains unmethylated CpG dinucleotides in the context of 
                     5'-AACGTT-3' (Sato et al., 1996)"
     primer_bind     complement(556..575)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 ctctcgccct tgcttgtgcc gtggtgatgg tggtgatggt ggtgaccaga ggtgcccatg
       61 gtttcactta cgtgtttaat gtctttacag actttataca ttactgtcat ggttaaataa
      121 aataaaataa aataaaataa aataaaataa aataaaataa aataaaataa acttaagatg
      181 tccctatagt gagtcgtatt agacgtcagg tggcactttt cggggaaatg tgcgcggaac
      241 ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc
      301 ctgataaatg cttcaataat attgaaaaag gaagagtatg agtattcaac atttccgtgt
      361 cgcccttatt cccttttttg cggcattttg ccttcctgtt tttgctcacc cagaaacgct
      421 ggtgaaagta aaagatgctg aagatcagtt gggtgcacga gtgggttaca tcgaactgga
      481 tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc caatgatgag
      541 cacttttaaa gttctgctat gtggcgcggt attatcccgt attgacgccg ggcaagagca
      601 actcggtcgc cgcatacact attctcagaa tgacttggtt gagtactcac cagtcacaga
      661 aaagcatctt acggatggca tgacagtaag agaattatgc agtgctgcca taaccatgag
      721 tgataacact gcggccaact tacttctgac aacgatcgga ggaccgaagg agctaaccgc
      781 ttttttgcac aacatggggg atcatgtaac tcgccttgat cgttgggaac cggagctgaa
      841 tgaagccata ccaaacgacg agcgtgacac cacgatgcct gtagcaatgg caacaacgtt
      901 gcgcaaacta ttaactggcg aactacttac tctagcttcc cggcaacaat taatagactg
      961 gatggaggcg gataaagttg caggaccact tctgcgctcg gcccttccgg ctggctggtt
     1021 tattgctgat aaatctggag ccggtgagcg tgggtc
//
