LOCUS       sequence-119683-        1088 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Standard for cell-free expression benchmarking..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1088)
  AUTHORS   Gagoski D, Polinkovsky ME, Mureev S, Kunert A, Johnston W, Gambin Y,
            Alexandrov K
  TITLE     Performance benchmarking of four cell-free protein expression 
            systems.
  JOURNAL   Biotechnol Bioeng. 2015 Aug 24. doi: 10.1002/bit.25814.
  PUBMED    26301602
REFERENCE   2  (bases 1 to 1088)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Biotechnol 
            Bioeng. 2015 Aug 24. doi: 10.1002/bit.25814."
COMMENT     Sequence Label: pCellFree_G03 GTF2H4
FEATURES             Location/Qualifiers
     source          1..1088
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    166..190
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 taccacgcgt acccaacaca cggacacatg cacgaacacg catgcagaga gatcatacat
       61 catctataga gaagtacacg taaaagtaag actcgtcgtc tgccagctac aaccgagaag
      121 gcgacgtgaa aggaacaaga aaggaggagg agggcggccg cttacaccac tttgtacaag
      181 aaagctgggt ccaaggagct atgtttctgc cgcttccaaa agcgcttgac gtcgctgtgc
      241 ccggccgggg tcaccaccat gagccgcttg gccgagttct cgaacacgag cacgcccagc
      301 tcccgcgcgt gggccagcag cagctcaaag tccacttgcg acaggaactg gttatacagg
      361 acaccctcag tgaaccggag tctgtccctt tccagctccc agagccggat ctggtcggtg
      421 atggtggggg gcagcacagg tgtctgtttg agcatcactg ggtgggctct tgtccttagg
      481 aaatggatta tctgctgggc tgtgatgcca ctggcgattg cctgctgcac actctcccgg
      541 gtcacctgcg ccaccaccat gttggggaac cgatagagca tctcagagaa gagggcaatg
      601 agggcaatct gcagctccga ctccgtgtag gcatacagtc ggtaattggt ttccacgaca
      661 atgaaacctg gctgatgcac agtgccccca gctccagaga cacctgatga gagattgatg
      721 gccaggcgtg tggggtagta acgccgagat ttcctcttcc tctggaaaac aagcccaaac
      781 tcacgcagat gttgcaggaa gttcaacaga gaatcactca taccttccac agagtaatcc
      841 ttgcccagag tagagaagct gagctggaag aggaaggaga gaatctctac caggtccatg
      901 ccccggctct gggctgtctg caaatactgc aacataaagt accagagctg agccggggtg
      961 tccagcaaca ggaactggaa gccagcggaa gtaatgcagg gcggctctcc aggttcagta
     1021 ctcttcatga gcccagcctg gctgaggagc tgagccaagt cctggctgac agctgcactg
     1081 ggggagcc
//
