LOCUS       sequence-119687-        1095 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Standard for cell-free expression benchmarking..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1095)
  AUTHORS   Gagoski D, Polinkovsky ME, Mureev S, Kunert A, Johnston W, Gambin Y,
            Alexandrov K
  TITLE     Performance benchmarking of four cell-free protein expression 
            systems.
  JOURNAL   Biotechnol Bioeng. 2015 Aug 24. doi: 10.1002/bit.25814.
  PUBMED    26301602
REFERENCE   2  (bases 1 to 1095)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Biotechnol 
            Bioeng. 2015 Aug 24. doi: 10.1002/bit.25814."
COMMENT     Sequence Label: pCellFree_G03 SMAD2
FEATURES             Location/Qualifiers
     source          1..1095
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    166..190
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 taccacgcgt acccaacaca cggacacatg cacgaacacg catgcagaga gatcatacat
       61 catctataga gaagtacacg taaaagtaag actcgtcgtc tgccagctac aaccgagaag
      121 gcgacgtgaa aggaacaaga aaggaggagg agggcggccg cttacaccac tttgtacaag
      181 aaagctgggt ccaatgacat gcttgagcaa cgcactgaag gggatcccat ctgagttaat
      241 actttgtcca accactgtag aggtccattc agatgaagtt caatccagca aggagtactt
      301 gttaccgtct gccttcggta ttctgctccc caccctttca caaaactcat tcttatggtg
      361 cacattctag ttagctgata gacggcttca aaaccctgat taacagactg agccagaaga
      421 gcagcaaatt cctggttgtt gaagatcttc agattacagc ctggtggaat tttacacact
      481 gttgcagggt gccagccata tctctgatta caattggggc tctgcacaaa gattgcacta
      541 tcacttaggc actcagcaaa aacttcccca cctatgtagt ataagcgcac tcctcttcct
      601 atatgccttc ttgtcatttc taccgtggca tttcggttaa cattggagag taaacctaag
      661 cagaacctct ctgaatttga tgggtctgta aagccatcta cagtgagtga gggctgtgat
      721 gcatggaagg tttctccaac cctctgattt aattcataat atgctatcga acaccaaaat
      781 gcaggttctg agtaagtaac tggctgtaaa tccaagctat gattaacagg ggaaagagta
      841 gtaggagata gttctgctgg agagcctgtg tccatacttt gattcaactg ttggtcactt
      901 gtttctccat cttcactgat atatccagga ggtggcgttt ctggaatata attactctgt
      961 ggctcaattc ctgctgggaa gttagtgttt tctggaatgg agtgagtata gtcatccaga
     1021 ggcggaagtt ctgttaggat ctcggtgtgt cggggcacta atactggagg caaaactggt
     1081 gtctcaactc tctga
//
