LOCUS       sequence-119688-         493 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Vector for Cas9 mRNA synthesis.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 493)
  AUTHORS   Harms DW, Quadros RM, Seruggia D, Ohtsuka M, Takahashi G, Montoliu 
            L, Gurumurthy CB
  TITLE     Mouse Genome Editing Using the CRISPR/Cas System.
  JOURNAL   Curr Protoc Hum Genet. 2014 Oct 1;83:15.7.1-15.7.27. doi: 
            10.1002/0471142905.hg1507s83.
  PUBMED    25271839
REFERENCE   2  (bases 1 to 493)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Curr Protoc
            Hum Genet."; date: "2014-10-1"; volume: "83:15.7.1-15.7.27. doi"; 
            pages: " 10.1002/0471142905.hg1507s83"
COMMENT     Sequence Label: pBGK
FEATURES             Location/Qualifiers
     source          1..493
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             272..319
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
ORIGIN
        1 gagagtgatc ctggccgacg ctaatctgga caaagtgctg tccgcctaca acaagcaccg
       61 ggataagccc atcagagagc aggccgagaa tatcatccac ctgtttaccc tgaccaatct
      121 gggagcccct gccgccttca agtactttga caccaccatc gaccggaaga ggtacaccag
      181 caccaaagag gtgctggacg ccaccctgat ccaccagagc atcaccggcc tgtacgagac
      241 acggatcgac ctgtctcagc tgggaggcga caaaaggccg gcggccacga aaaaggccgg
      301 ccaggcaaaa aagaaaaagt aagaattctg cagatatcca gcacagtggc ggccgctcga
      361 ctatgggcac caaagaacct gtaaacgtta tctttttaaa ttgaatgtgc acaaataaaa
      421 gtttggaaaa gaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa
      481 aaaaaaaaaa aaa
//
