LOCUS       sequence-119689-         947 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  A catalytically inactive SaCas9 (dSaCas9) with the D10A and N580A 
            mutations. .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 947)
  AUTHORS   Ran FA, Cong L, Yan WX, Scott DA, Gootenberg JS, Kriz AJ, Zetsche B,
            Shalem O, Wu X, Makarova KS, Koonin EV, Sharp PA, Zhang F
  TITLE     In vivo genome editing using Staphylococcus aureus Cas9.
  JOURNAL   Nature. 2015 Apr 1. doi: 10.1038/nature14299.
  PUBMED    25830891
REFERENCE   2  (bases 1 to 947)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nature. 
            2015 Apr 1. doi: 10.1038/nature14299."
COMMENT     Sequence Label: 
            pX603-AAV-CMV::NLS-dSaCas9(D10A,N580A)-NLS-3xHA-bGHpA
FEATURES             Location/Qualifiers
     source          1..947
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 tggtcttgct gattctgccc ttgcccttgg ccagattcag gatgtgcttc ttgaaggttt
       61 cgtagctgat cttgctgtcg ctgctgctca ggtactggaa tggggtccgg ttgcccttct
      121 tgctggcttc ttcctgcttc acgagcacct tgttgttgaa gctgttgtcg aaggacacgc
      181 ttctggggat gatgtggtcc acctcatagt tgaaggggtt gttcagcaga tcttccagag
      241 ggatggcttc caggctgtac aggcacttgc cttcctgcat gtcgtgcagc ttgatcttct
      301 cgatcaggta cttggcgttc tctttgccgg tggtccggat gatttcctcg atccgctcgt
      361 tggtctgccg gttccgcttc tgcatctcgt tgatcatttt ctgggcgtcc ttggagttct
      421 tctcgcgggc cagctcgata atgatgtcgt tgggcaggcc gtacttcttg atgatggcgt
      481 tgatcacttt gatgctctgg atgaagcttc tcttcacgac ggggctcagg atgaagtcgt
      541 ccaccagggt ggtggggatc tctttctgct gggacaggtc caccttcttg ggcaccagct
      601 tcagccggtt gaagatagcg atctggttgt cgttggtgtg ccacagctcg tccaggatca
      661 ggttgatggc cttcaggctc aggttgtggg tgccggtata gcccttcaga ttagagatct
      721 gctcgatctc ttcctgggtc agctcggagt tcagattggt cagttcttcc tggatgtcct
      781 cgctgctctg gtagatggtc aggatcttgg caatctgatc cagcagctcg gcgttctcaa
      841 taatctcttt ccgggcggta atgtccttga tgtcgtggta caccttcagg ttggtgaact
      901 cgggcttgcc ggtgctggtc actctgtagc ccttaatatc ctcttcg
//
