LOCUS       sequence-119691-        1025 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Standard for cell-free expression benchmarking..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1025)
  AUTHORS   Gagoski D, Polinkovsky ME, Mureev S, Kunert A, Johnston W, Gambin Y,
            Alexandrov K
  TITLE     Performance benchmarking of four cell-free protein expression 
            systems.
  JOURNAL   Biotechnol Bioeng. 2015 Aug 24. doi: 10.1002/bit.25814.
  PUBMED    26301602
REFERENCE   2  (bases 1 to 1025)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Biotechnol 
            Bioeng. 2015 Aug 24. doi: 10.1002/bit.25814."
COMMENT     Sequence Label: pCellFree_G03 GTF2A1L
FEATURES             Location/Qualifiers
     source          1..1025
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    170..194
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 ggtaccacgc gtacccaaca cacggacaca tgcacgaaca cgccatgcag agagatcata
       61 catcatctat agagaagtac acgtaaaagt aagactcgct cgtctgccag ctacaaccga
      121 gaaggcgacg tgaaaggaac aagaaaggag gaggagggcg gccgcttaca ccactttgta
      181 caagaaagtt gggtaccact ctgcatcacc aatggctttt gcaaatacat agtctctccc
      241 tccaaaacac ataacaccat ctttcaaata gaatttccat ttgttcttgc ttcgatgaat
      301 cttatcatac tgacagacaa taacattatc cgtgtcaaac aggtctggca catcctgttc
      361 actaacatca tctccagaat ttaaagggtc ctcttctaca atgtttactt gagggtcttc
      421 attatcacta ctgtttgtgg ctgaatcctc atttgaaata ctgtcagctt cttcttcagg
      481 taccttcaga tctcccccgt caatattccc tagaaattca ttctcatctg catctcttgt
      541 acttcctatt tcttcattgg aagatgtatc accgcttcca tctacttgaa ttatttcacc
      601 aatatcatca tcagtaaccc gaatgcttaa atccacctga gaattagaat ccttctctga
      661 tacaggtatg ttgctgggtt cctctatatt tcttggttgc tttacagaat cacatccata
      721 catcctattt ttaagaatat gaagctgaat atcagtcacg tgctggtgga gagccccatg
      781 agggcttgtg gagagggact catcatgcag attttgagcc atgctagctg aaccactgag
      841 cactgactcc acattagaat ttgtttgaga gacctgtgga gaaaatacaa cacctggaag
      901 actgatatag tgagaacttt cctgatgaca caacaaagct tcaggcacga ttttatgctc
      961 atttccagga gatacaagta tatcactggt ggcattttct aagtgtttcc tatctactgg
     1021 cccaa
//
