LOCUS       sequence-119697-         340 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  sgRNA for Pten deletion.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 340)
  AUTHORS   Li Y, Park A, Mou H, Colpan C, Bizhanova A, Akama-Garren E, Joshi N,
            Hendrickson EA, Feldser D, Yin H, Anderson DG, Jacks T, Weng Z, Xue 
            W
  TITLE     A versatile reporter system for CRISPR-mediated chromosomal 
            rearrangements.
  JOURNAL   Genome Biol. 2015 May 28;16(1):111.
  PUBMED    26018130
REFERENCE   2  (bases 1 to 340)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Genome 
            Biol."; date: "2015-05-28"; volume: "16(1)"; pages: "111"
COMMENT     Sequence Label: pX330.Pten.a
FEATURES             Location/Qualifiers
     source          1..340
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     62..81
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     misc_RNA        161..236
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
ORIGIN
        1 tgacgctaga aagtaataat ttcttgggta gtttgcagtt ttaaaattat gttttaaaat
       61 ggactatcat atgcttaccg taacttgaaa gtatttcgat ttcttggctt tatatatctt
      121 gtggaaagga cgaaacaccg tttcatagcg gccacgaagt gttttagagc tagaaatagc
      181 aagttaaaat aaggctagtc cgttatcaac ttgaaaaagt ggcaccgagt cggtgctttt
      241 ttgttttaga gctagaaata gcaagttaaa ataaggctag tccgttttta gcgcgtgcgc
      301 caattctgca gacaaatggc tctagaggta cccgttacat
//
