LOCUS       sequence-119699-         411 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  sgRNA for Pten deletion.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 411)
  AUTHORS   Li Y, Park A, Mou H, Colpan C, Bizhanova A, Akama-Garren E, Joshi N,
            Hendrickson EA, Feldser D, Yin H, Anderson DG, Jacks T, Weng Z, Xue 
            W
  TITLE     A versatile reporter system for CRISPR-mediated chromosomal 
            rearrangements.
  JOURNAL   Genome Biol. 2015 May 28;16(1):111.
  PUBMED    26018130
REFERENCE   2  (bases 1 to 411)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Genome 
            Biol."; date: "2015-05-28"; volume: "16(1)"; pages: "111"
COMMENT     Sequence Label: pX330.Pten.b
FEATURES             Location/Qualifiers
     source          1..411
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     112..131
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     misc_RNA        210..285
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
ORIGIN
        1 ataattggaa ttaatttgat gctaaacaaa agatattagt acacaaatac gtgacgtaga
       61 aagtaataat ttcttgggta gtttgcagtt ttaaaattat gttttaaaat ggactatcat
      121 atgcttaccg taacttgaaa gtatttcgat ttcttggctt tatatatctt gtggaaagga
      181 cgaaacaccg ctgtagtaat atctgctatg ttttagagct agaaatagca agttaaaata
      241 aggctagtcc gttatcaact tgaaaaagtg gcaccgagtc ggtgcttttt tgttttagag
      301 ctagaaatag caagttaaaa taaggctagt ccgtttttag cgcgtgcgcc aattctgcag
      361 acaaatggct ctagaggtac ccgttacata acttacggta aatggcccgc c
//
