LOCUS       sequence-119703-        1021 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  co-expression of Cas9 and a sgRNA targeting K08F4.2 in the middle of
            the ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1021)
  AUTHORS   Paix A, Wang Y, Smith HE, Lee CY, Calidas D, Lu T, Smith J, Schmidt 
            H, Krause MW, Seydoux G
  TITLE     Scalable and versatile genome editing using linear DNAs with 
            microhomology to Cas9 Sites in Caenorhabditis elegans.
  JOURNAL   Genetics. 2014 Dec;198(4):1347-56. doi: 10.1534/genetics.114.170423.
            Epub 2014 Sep 23.
  PUBMED    25249454
REFERENCE   2  (bases 1 to 1021)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1534/genetics.114.170423"; journalName: "Genetics"; date: 
            "2014-12"; volume: "198"; issue: "4"; pages: "1347-56"
COMMENT     Sequence Label: APq5271
FEATURES             Location/Qualifiers
     source          1..1021
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    23..43
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     promoter        44..643
                     /gene="eft-3"
                     /label=eft-3 promoter
                     /note="promoter for the C. elegans elongation factor 
                     1-alpha gene (Mitrovich and Anderson, 2000)"
ORIGIN
        1 gaccatgatt acgccaagct atcaactttg tatagaaaag ttggcacctt tggtctttta
       61 ttgtcaactt ccattggttc ttccattgtt tctgttaaat taatgaattt ttcataaaat
      121 aaagacatta tacaatataa aaatgaagaa tttattgaaa ataaactgcc agagagaaaa
      181 agtatgcaac actcccgccg agagtgtttg aaatggtgta cggtacattt tcgtgctagg
      241 agttagatgt gcaggcagca acgagagggg gagagatttt tttgggcctt gtgaaattaa
      301 cgtgagtttt ctggtcatct gactaatcat gttggttttt tgttggttta ttttgttttt
      361 atctttgttt ttatccagat taggaaattt aaattttatg aatttataat gaggtcaaac
      421 attcagtccc agcgtttttc ctgttctcac tgtttagtcg aatttttatt ttaggctttc
      481 aacaaatgtt ctaactgtct tatttgtgac ctcacttttt atattttttt aatttttaaa
      541 aatattagaa gtttctagga taattttttc gacttttatt ctctctaccg tccgcactct
      601 tcttactttt aaattaaatt gttttttttt cagttgggaa acactttgct ctagaaaaaa
      661 tggacaaaaa atacagcatc ggccttgaca tcggaacgaa ctccgtcgga tgggccgtca
      721 tcaccgacga gtacaaggtc ccatccaaga agttcaaggt cctcggaaac accgaccgtc
      781 actccatcaa gaagaacctc atcggagccc tcctcttcga ctccggagag accgccgagg
      841 ccacccgtct caagcgtacc gcccgtcgtc gttacacccg tcgtaagaac cgtatctgct
      901 acctccaaga gatcttctcc aacgagatgg ccaaggtcga cgactccttc ttccaccgtc
      961 tcgaggagtc cttcctcgtc gaggaggaca agaagcacga gcgtcaccca atcttcggaa
     1021 a
//
