LOCUS       sequence-119704-         353 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  sgRNA for iGFP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 353)
  AUTHORS   Li Y, Park A, Mou H, Colpan C, Bizhanova A, Akama-Garren E, Joshi N,
            Hendrickson EA, Feldser D, Yin H, Anderson DG, Jacks T, Weng Z, Xue 
            W
  TITLE     A versatile reporter system for CRISPR-mediated chromosomal 
            rearrangements.
  JOURNAL   Genome Biol. 2015 May 28;16(1):111.
  PUBMED    26018130
REFERENCE   2  (bases 1 to 353)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Genome 
            Biol."; date: "2015-05-28"; volume: "16(1)"; pages: "111"
COMMENT     Sequence Label: pX330.iGFP2
FEATURES             Location/Qualifiers
     source          1..353
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     52..71
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     misc_RNA        150..225
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
ORIGIN
        1 aagtaataat ttcttgggta gtttgcagtt ttaaaattat gttttaaaat ggactatcat
       61 atgcttaccg taacttgaaa gtatttcgat ttcttggctt tatatatctt gtggaaagga
      121 cgaaacaccg agcggccgct cgagtctagg ttttagagct agaaatagca agttaaaata
      181 aggctagtcc gttatcaact tgaaaaagtg gcaccgagtc ggtgcttttt tgttttagag
      241 ctagaaatag caagttaaaa taaggctagt ccgtttttag cgcgtgcgcc aattctgcag
      301 acaaatggct ctagaggtac ccgttacata acttacggta aatggcccgc ctg
//
