LOCUS       sequence-119711-        1077 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Standard for cell-free expression benchmarking..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1077)
  AUTHORS   Gagoski D, Polinkovsky ME, Mureev S, Kunert A, Johnston W, Gambin Y,
            Alexandrov K
  TITLE     Performance benchmarking of four cell-free protein expression 
            systems.
  JOURNAL   Biotechnol Bioeng. 2015 Aug 24. doi: 10.1002/bit.25814.
  PUBMED    26301602
REFERENCE   2  (bases 1 to 1077)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Biotechnol 
            Bioeng. 2015 Aug 24. doi: 10.1002/bit.25814."
COMMENT     Sequence Label: pCellFree_G03 SP6
FEATURES             Location/Qualifiers
     source          1..1077
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    167..191
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 taccacgcgt acccaacaca cggacacatt gcacgaacac gcatgcagag agatcataca
       61 tcatctatag agaagtacac gtaaaagtaa gactcgtcgt ctgccagcta caaccgagaa
      121 ggcgacgtga aaggaacaag aaaggaggag gagggcggcc gcttacacca ctttgtacaa
      181 gaaagttggc aagttggagg gagccacgct gccctcggcc tcgcgtttgc ctttgccccc
      241 ggggggctcc actgcgccgc cggccttgcc ctctcccgag gccgccccag ccgcctcctc
      301 cttggcgccc tcgtgggttt tcatgtgctt ggccaggtgg tcgctgcgca tgaagacgcg
      361 gctgcagact gcacagggga acttcttggt gccggtgtgg gtctggaggt ggcgctgcag
      421 ctcgtccgag cgcgtgaagc gcttgccgca gaagagccag ttgcacacga agggacggtc
      481 gccgctgtgc cagcgcaggt gcgccttcag gtgcgacgtc ttggcgtagg ctttcccgca
      541 gcccgggatg tggcagttgt gcaaatgctt cttcttgccc ccatcgggcc cacatggagc
      601 ccccagtcgc tccgcctcca gacagttggg gcagcgacag acggtctggc ctgagctgcg
      661 gggcaccgac cgccgggagc ctttgggacg cgccgccccg tccaggctgg aatccagccc
      721 ttgagactcc ggggcggcta cttccaaggc cttagccccg tcgggcggcc ctaggagatg
      781 ctgccctccg gcagctggaa ggaggtggtg cgcatgcggg tggggtggcg gggcacaaag
      841 ctggtggtct ccgacgtagc cccccaagcc cgcctgaagc gcccccgggt ggccaggtga
      901 ggtcagcgcg ccctgagtgt gggggaggtc catccagctg gtgcccggat gaaggtccca
      961 ccacgagcca tcctccgcgc ctgggtgagt cggcctgaac cacgattcat aatggtgtga
     1021 catgtccggc tgcaggagct tggaaaaggg gcccggggcc aagggactgt cgctttc
//
