LOCUS       sequence-119716-        1049 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Standard for cell-free expression benchmarking..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1049)
  AUTHORS   Gagoski D, Polinkovsky ME, Mureev S, Kunert A, Johnston W, Gambin Y,
            Alexandrov K
  TITLE     Performance benchmarking of four cell-free protein expression 
            systems.
  JOURNAL   Biotechnol Bioeng. 2015 Aug 24. doi: 10.1002/bit.25814.
  PUBMED    26301602
REFERENCE   2  (bases 1 to 1049)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Biotechnol 
            Bioeng. 2015 Aug 24. doi: 10.1002/bit.25814."
COMMENT     Sequence Label: pCellFree_G03 CAMK1
FEATURES             Location/Qualifiers
     source          1..1049
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    166..190
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             645..656
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
ORIGIN
        1 taccacgcgt acccaacaca cggacacatg cacgaacacg catgcagaga gatcatacat
       61 catctataga gaagtacacg taaaagtaag actcgtcgtc tgccagctac aaccgagaag
      121 gcgacgtgaa aggaacaaga aaggaggagg agggcggccg cttacaccac tttgtacaag
      181 aaagttggca agagctggtg gggcagtgtg ggggacagtt ctgtgcccgg ctccacgcag
      241 cagtctcgac agcaacagcc agctgccggc cccccagcca ctggtgtcag cagctcccca
      301 tggctcgccg tctgcccctg cccctcctgg ctggtgccca gctgcagttt cctcatgtgc
      361 cgcaccacag ccgtggcatt gaaggcttgc ttccacttgc tcttggcaaa gttcttcttg
      421 atctgctcac tcaccgactg gtggatattc ttatctagag ctgtatctcc tgcaatccat
      481 gggtgctgca aggcctgctc acaggtgaat cttttctctg ggtccttctc catcaagtgc
      541 cggatgaaat ctttggcaga gtcagagatg tcgtcccagt aaggagagtc aaactcgtac
      601 tcggccttca aaatctgttc aaagagtttg gcatcattct cgtcatagaa gggagggtaa
      661 ccgcagagca agatgtaggc gatgacacct atggaccagc aatccacagc cttgctgtag
      721 ggcttctggg ccaggacttc aggggccacg tatcccggag ttccacaggc ggtggagagc
      781 acactgcccg ggtcctccat cttggagagg ccaaagtcgg agatcatgat tttggagtct
      841 tcatccaggc tgtagtacag cagattctct ggcttgagat cccggtgtac aatgcccagg
      901 tcatgcaggt atttcacagc atccagcacc tggaagatga ggcggctggc gtcccgctcc
      961 gtgtagaagc ctttttccac aatacggtca aagagctccc cacccgacac cagctgcatg
     1021 atgaggtaga ggtggccccc actctcata
//
