LOCUS       sequence-119718-        1060 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Standard for cell-free expression benchmarking..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1060)
  AUTHORS   Gagoski D, Polinkovsky ME, Mureev S, Kunert A, Johnston W, Gambin Y,
            Alexandrov K
  TITLE     Performance benchmarking of four cell-free protein expression 
            systems.
  JOURNAL   Biotechnol Bioeng. 2015 Aug 24. doi: 10.1002/bit.25814.
  PUBMED    26301602
REFERENCE   2  (bases 1 to 1060)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Biotechnol 
            Bioeng. 2015 Aug 24. doi: 10.1002/bit.25814."
COMMENT     Sequence Label: pCellFree_G03 GTF2A1
FEATURES             Location/Qualifiers
     source          1..1060
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    167..191
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 taccacgcgg tacccaacac acggacacat gcacgaacac gcatgcagag agatcataca
       61 tcatctatag agaagtacac gtaaaagtaa gactcgtcgt ctgccagcta caaccgagaa
      121 ggcgacgtga aaggaacaag aaaggaggag gagggcggcc gcttacacca ctttgtacaa
      181 gaaagttggc aaccattctg catctccaat ggctttggaa aatatataat ctcttccatt
      241 aagattcata atgccatcct tgagatgaaa tttccatttg tttttacttc tgtgtatctt
      301 atcatattgg catacaacaa cattttctgt gtcaaagagt tcctgtcctt cctcatcact
      361 cacatcatct tcactattga ggggctcttc ttccacctgc ccatcttcag ctccatcttt
      421 ctctttgtct tcctcctcat catcatcata gtcttcttct tcatcttcat cttcttcaga
      481 tgatgtatcc ccagttccat caacttgtaa gaccaatgga gcttgtgttt gagcaggctg
      541 ggcctgcggt tgctgctggc cagttgcagt tatctgtgct tgggctggtg taggtgctgc
      601 cactgtcgta ggtataactt gagtcttatt tcctgtaaat aagatttgct gaggctggat
      661 gatgacacct gtctgtggtg aaatccctcc aggaagagga gccagcacct gctgtataac
      721 aggagcttgt actccaccag gctgcatttg tggtataacc tgttgttgta gaaccactga
      781 ctgctgaggc tgaaagatat attgggcacc attggctgct ctgaccacct gaagaagctg
      841 gcctgaattt gttaatatct gctgaacagg agtcacacct gcagggagtg ctaaggtagc
      901 agctgtagca gcagcactca tgtttgatgc attcatatgc tgtatcaact tagaatctgg
      961 aacaataact tgtggtgctg tggcttgctg tgatgcagga ataagaacct gctgggtctg
     1021 cgcttgctga ggtactgtct gctgaggctg agcttgctga
//
