LOCUS       sequence-119720-        1000 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Standard for cell-free expression benchmarking..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1000)
  AUTHORS   Gagoski D, Polinkovsky ME, Mureev S, Kunert A, Johnston W, Gambin Y,
            Alexandrov K
  TITLE     Performance benchmarking of four cell-free protein expression 
            systems.
  JOURNAL   Biotechnol Bioeng. 2015 Aug 24. doi: 10.1002/bit.25814.
  PUBMED    26301602
REFERENCE   2  (bases 1 to 1000)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Biotechnol 
            Bioeng. 2015 Aug 24. doi: 10.1002/bit.25814."
COMMENT     Sequence Label: pCellFree_G03 MAPK13
FEATURES             Location/Qualifiers
     source          1..1000
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    167..191
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 taccacgcgt acccaacaca cggacacatg cacgaacacg catgcagaga gatcatacat
       61 catctataga gaagtacacg taaaagtaag actcgctcgt ctgccagcta caaccgagaa
      121 ggcgacgtga aaggaacaag aaaggaggag gagggcggcc gcttacacca ctttgtacaa
      181 gaaagttggg tacagcttca tgccactccg gcgccgtgag tccttccggg caatggggct
      241 gaagttcaca atctccttgt agatgtgctg cttccattca tccactgtga gtttctcgtg
      301 ttctaaggaa tcatcaaacg gctgctgggc ctccgtctct tcctcagggt cccggaaggg
      361 ttcaaagaag ggatgggtga gggcctgcgc ggccgtcagg cgcttgtcca cgtctagctc
      421 cagcatcttc tccagcaggt ccgcagcctg ggggctggcc cgtgggaaca gctgagtgaa
      481 atccttcctg ggggtctgtg gcagggactg gatgtaggat ttggccgctt tgtcgttcag
      541 cttctgcaca aactccgtgc caggcacccc ggtcactttc aggatctggg tcagctggtc
      601 caggtaatct ttccccttga acagagtttt ccctgtcagc atctctgcca tgatacagcc
      661 cacagaccag atgtccactg tctggttgta gtgcatccag ctgaggatca cctcgggggc
      721 tcggtaccag cgggtcacca cgtagccagt catctcggcg tctgcatgtc gcgccagccc
      781 aaaatccaga atcttcagtt cacagtcctc attcacagcc aggttgcctg gcttcaggtc
      841 cctgtgcacg accccagcag agtggatgta cttaaggcct ttgagcatct gatacaccag
      901 gtactggatc ttctcctcac tgaactccat ccccatgatc ttctgcagat ccgtctgcat
      961 gaagggcatc accaggtaga agtcataaaa gttgcgcagg
//
