LOCUS       sequence-119722-         875 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Nucleus/Histones, Excitation: 485, Emission: 507.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 875)
  TITLE     Davidson Green fluorescent proteins
REFERENCE   2  (bases 1 to 875)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: sfGFP-H2B-6
FEATURES             Location/Qualifiers
     source          1..875
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             83..460
                     /codon_start=1
                     /gene="HIST1H2BJ"
                     /product="human histone H2B"
                     /label=H2B
                     /translation="MPEPAKSAPAPKKGSKKAVTKAQKKGGKKRKRSRKESYSIYVYKV
                     LKQVHPDTGISSKAMGIMNSFVNDIFERIAGEASRLAHYNKRSTITSREIQTAVRLLLP
                     GELAKHAVSEGTKAITKYTSAK"
     regulatory      473..482
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(524..545)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
ORIGIN
        1 tgaaccgtca gaatccgcta gcgctaccgg actcagatct cgagctcaag cttcgaattc
       61 tgcagtcgac ggtaccgcca ccatgccaga gccagcgaag tctgctcccg ccccgaaaaa
      121 gggctccaag aaggcggtga ctaaggcgca gaagaaaggc ggcaagaagc gcaagcgcag
      181 ccgcaaggag agctattcca tctatgtgta caaggttctg aagcaggtcc accctgacac
      241 cggcatttcg tccaaggcca tgggcatcat gaattcgttt gtgaacgaca ttttcgagcg
      301 catcgcaggt gaggcttccc gcctggcgca ttacaacaag cgctcgacca tcacctccag
      361 ggagatccag acggccgtgc gcctgctgct gcctggggag ttggccaagc acgccgtgtc
      421 cgagggtact aaggccatca ccaagtacac cagcgctaag gatccaccgg tcgccaccat
      481 ggtgagcaag ggcgaggagc tgttcaccgg ggtggtgccc atcctggtcg agctggacgg
      541 cgacgtaaac ggccacaagt tcagcgtgcg cggcgagggc gagggcgatg ccaccaacgg
      601 caagctgacc ctgaagttca tctgcaccac cggcaagctg cccgtgccct ggcccaccct
      661 cgtgaccacc ctgacctacg gcgtgcagtg cttcagccgc taccccgacc acatgaagcg
      721 ccacgacttc ttcaagtccg ccatgcccga aggctacgtc caggagcgca ccatcagctt
      781 caaggacgac ggcacctaca agacccgcgc cgaggtgaag ttcgagggcg acaccctggt
      841 gaaccgcatc gagctgaagg gcatcgactt caagg
//
