LOCUS       sequence-119723-        1008 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Standard for cell-free expression benchmarking..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1008)
  AUTHORS   Gagoski D, Polinkovsky ME, Mureev S, Kunert A, Johnston W, Gambin Y,
            Alexandrov K
  TITLE     Performance benchmarking of four cell-free protein expression 
            systems.
  JOURNAL   Biotechnol Bioeng. 2015 Aug 24. doi: 10.1002/bit.25814.
  PUBMED    26301602
REFERENCE   2  (bases 1 to 1008)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Biotechnol 
            Bioeng. 2015 Aug 24. doi: 10.1002/bit.25814."
COMMENT     Sequence Label: pCellFree_G03 IRF9
FEATURES             Location/Qualifiers
     source          1..1008
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    167..191
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 taccacgcgt acccaacaca cggacacatg cacgaacacg ccatgcagag agatcataca
       61 tcatctatag agaagtacac gtaaaagtaa gactcgtcgt ctgccagcta caaccgagaa
      121 ggcgacgtga aaggaacaag aaaggaggag gagggcggcc gcttacacca ctttgtacaa
      181 gaaagttggg tacaccggga cagaatggct gcctgctgct ctggagtctg ctccagcaag
      241 tatcgggcaa aggcctgctc catcttcact gtgataagat tctgtggagt atggctggag
      301 ccatggctct cttcccagaa attcagtgtt acctggaact tcggtggggg gcccaggccc
      361 tgaaagtacc tgaccaagtc tctgcagaag taggcggttc tgaagagctc cacgcactcg
      421 ttgctgggca gcagatgcgg gcctggccca ggtggagcct ggggtgcatt ccaggagatg
      481 gggatggggc aaaggcgctg cacgaagagg cctcgggggt tgctggccac taggatgccc
      541 ctctcaagct ggctcagcag gcgctgcgtg ggctccagtg ggccaggctt ggggaacagc
      601 acctgctcca tgctgctctc agagcctgag ggctcagcca caaggcggca atccaggctt
      661 tgcacctggg cctcgcccac cacgcgcccg ttgtagatga aggtgagcag cagtgagtag
      721 tctggctctg gaggaagcag aaactccagg gacctctgat ccccttgaaa gggggcctca
      781 gttgtgtctg taacttcctg tggctcaggg ctgctgctgc tgctgctgct cccaatgtct
      841 gaatggactg ctcccccact ggccccctcc tcctcattat tgagggagtc ctggagcaca
      901 gagggactga gtgtgcagtt ctgcatggca tcctcttcct ccttcctctc agaggacaca
      961 gaactgtgct gtcgctttga tggtactttc tgagtccctg gctggcca
//
