LOCUS       sequence-119724-        1107 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expression of human SNRNP70 fused to Flag-HA tag (N-terminus).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1107)
  AUTHORS   Beck DB, Narendra V, Drury WJ 3rd, Casey R, Jansen PW, Yuan ZF, 
            Garcia BA, Vermeulen M, Bonasio R
  TITLE     In Vivo Proximity Labeling for the Detection of Protein-Protein and 
            Protein-RNA Interactions.
  JOURNAL   J Proteome Res. 2014 Dec 5;13(12):6135-43. doi: 10.1021/pr500196b. 
            Epub 2014 Oct 29.
  PUBMED    25311790
REFERENCE   2  (bases 1 to 1107)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1021/pr500196b";
            journalName: "J Proteome Res"; date: "2014-12-5- 5"; volume: "13"; 
            issue: "12"; pages: "6135-43"
COMMENT     Sequence Label: pINTO-NFH::hSNRNP70
FEATURES             Location/Qualifiers
     source          1..1107
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    18..36
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    39..57
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     67..91
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     regulatory      174..183
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             183..206
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             216..242
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             249..269
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
ORIGIN
        1 ctatataagc agagctctcc ctatcagtga tagagatctc cctatcagtg atagagatcg
       61 tcgacgagct cgtttagtga accgtcagat cgcctggaga cgccatccac gctgttttga
      121 cctccataga agacaccggg accgatccag cctccggact ctagcgttta aaggccacca
      181 tggattacaa ggatgacgac gataagggct ccggataccc atacgatgtg ccagattacg
      241 ctggcagcga gaacctgtac ttccagggct ccggaaccgg tagcggtacc gagctcggat
      301 ccatgaccca gttcctgccg cccaaccttc tggccctctt tgccccccgt gaccctattc
      361 catacctgcc acccctggag aaactgccac atgaaaaaca ccacaatcaa ccttattgtg
      421 gcattgcgcc gtacattcga gagtttgagg acccccgaga tgcccctcct ccaactcgtg
      481 ctgaaacccg agaggagcgc atggagagga aaagacggga aaagattgag cggcgacagc
      541 aagaagtgga gacagagctt aaaatgtggg accctcacaa tgatcccaat gctcaggggg
      601 atgccttcaa gactctcttc gtggcgagag tgaattatga cacaacagaa tccaagctcc
      661 ggagagagtt tgaggtgtac ggacctatca aaagaataca catggtctac agtaagcggt
      721 caggaaagcc ccgtggctat gccttcatcg agtacgaaca cgagcgagac atgcactccg
      781 cttacaaaca cgcagatggc aagaagattg atggcaggag ggtccttgtg gacgtggaga
      841 ggggccgaac cgtgaagggc tggaggcccc ggcggctagg aggaggcctc ggtggtacca
      901 gaagaggagg ggctgatgtg aacatccggc attcaggccg cgatgacacc tcccgctacg
      961 atgagaggcc cggcccctcc ccgcttccgc acagggaccg ggaccgggac cgtgagcggg
     1021 agcgcagaga gcggagccgg gagcgagaca aggagcgaga acggcgacgc tcccgctccc
     1081 gggaccggcg gaggcgctca cggagtc
//
