LOCUS       sequence-119729-        1018 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Standard for cell-free expression benchmarking..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1018)
  AUTHORS   Gagoski D, Polinkovsky ME, Mureev S, Kunert A, Johnston W, Gambin Y,
            Alexandrov K
  TITLE     Performance benchmarking of four cell-free protein expression 
            systems.
  JOURNAL   Biotechnol Bioeng. 2015 Aug 24. doi: 10.1002/bit.25814.
  PUBMED    26301602
REFERENCE   2  (bases 1 to 1018)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Biotechnol 
            Bioeng. 2015 Aug 24. doi: 10.1002/bit.25814."
COMMENT     Sequence Label: pCellFree_G03 TGFB1
FEATURES             Location/Qualifiers
     source          1..1018
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    169..193
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 taccacgcgt acccaacaca cggtacacat gcacgaacac gcatgcagag agcatcatac
       61 atcattctat agagaagtac acgtaaaagt aagactcgtc gtctgccagc tacaaccgag
      121 aaggcgacgt gaaaggaaca agaaaggagg aggagggcgg ccgcttacac cactttgtac
      181 aagaaagttg ggcagctgca cttgcaggag cgcacgatca tgttggacag ctgctccacc
      241 ttgggcttgc ggcccacgta gtacacgatg ggcagcggct ccagcgcctg cggcacgcag
      301 cacggcgccg ccgaggcgcc cgggttatgc tggttgtaca gggccaggac cttgctgtac
      361 tgcgtgtcca ggctccaaat gtaggggcag ggcccgaggc agaagttggc atggtagccc
      421 ttgggctcgt ggatccactt ccagccgagg tccttgcgga agtcaatgta cagctgccgc
      481 acgcagcagt tcttctccgt ggagctgaag caatagttgg tgtccagggc tcggcggtgc
      541 cgggagcttt gcagatgctg ggccctctcc agcggggtgg ccatgagaag caggaaaggc
      601 cggttcatgc catgaatggt ggccaggtca cctcggcggc cggtagtgaa cccgttgatg
      661 tccacttgca gtgtgttatc cctgctgtca caggagcagt gggcgctaag gcgaaagccc
      721 tcaatttccc ctccacggct caaccactgc cgcacaactc cggtgacatc aaaagataac
      781 cactctggcg agtcgctggg tgccagcagc cggttgctga ggtatcgcca ggaattgttg
      841 ctgtatttct ggtacagctc cacgtgctgc tccactttta acttgagcct cagcagacgc
      901 agctctgccc gggagagcaa cacgggttca ggtaccgctt ctcggagctc tgatgtgttg
      961 aagaacatat atatgctgtg tgtactctgc ttgaacttgt catagatttc gttgtggg
//
