LOCUS       sequence-119731-        1076 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Standard for cell-free expression benchmarking..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1076)
  AUTHORS   Gagoski D, Polinkovsky ME, Mureev S, Kunert A, Johnston W, Gambin Y,
            Alexandrov K
  TITLE     Performance benchmarking of four cell-free protein expression 
            systems.
  JOURNAL   Biotechnol Bioeng. 2015 Aug 24. doi: 10.1002/bit.25814.
  PUBMED    26301602
REFERENCE   2  (bases 1 to 1076)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Biotechnol 
            Bioeng. 2015 Aug 24. doi: 10.1002/bit.25814."
COMMENT     Sequence Label: pCellFree_G03 GTF2H2D
FEATURES             Location/Qualifiers
     source          1..1076
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    167..191
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 taccacgcgt acccaacaca cggacacatg cacgaacacg catgcagaga gatcatacat
       61 catctataga gaagtacacg taaaagtaag actcgctcgt ctgccagcta caaccgagaa
      121 ggcgacgtga aaggaacaag aaaggaggag gagggcggcc gcttacacca ctttgtacaa
      181 gaaagttggg caaacacctg aaggagctgg aatcttatga atacagccag ggcaacagtg
      241 tagagaatca tgaacaaaaa catcacagtc cacacagaaa acattttggc acacagcaca
      301 aacataaaca tgttggtctt tcaattcccc ctgacatcca taacaaaatc tttctccatt
      361 atattcttct aggggaattt cttgaaaagc atccaaagga aacaaatgat ggtaagaccg
      421 tgccaagtgg ggagcagaca ccaaagtaag accacagatt ttacattcaa caggtagctc
      481 acagtacttt gcccgacact gtgggcagaa atagcctcct aatgtaagcc ctggctcagt
      541 attgccatcc aaatgcgcca tgctgaaaga gggttttgca tcctggtcag ataaagaagc
      601 aatggtgtgc tgaggaaatc ccatacgaat aagtgagcat tcagaacttg agctagcagg
      661 aggaggacta agatgatgtg tgagcaactc tttgtaatgg ctttcatcta aaataacatg
      721 gtacgtgcca ccagtttcac gagcaagtac agtgcaaacg cgaacttctg cagacaatcc
      781 aataacagat actctaattt tagctgcctt tagggtcttg attaaatcat aaatattaga
      841 tggatcgcaa gttgtaaggc tgctaaagat gattagtact tctcgacttg tatgtccagg
      901 catgtgtttt agagtctgca tagccatgct tagggaatta taaagagatg gctctccatg
      961 gcaggtcata tccacagctt ccttcaaaga cgttatatgt tttcttgggt ttcctgaaag
     1021 ttcagtcaat ttttcagctc ttttactctt agttacaatt attccaatct gactaa
//
