LOCUS       sequence-119734-        1001 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Standard for cell-free expression benchmarking..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1001)
  AUTHORS   Gagoski D, Polinkovsky ME, Mureev S, Kunert A, Johnston W, Gambin Y,
            Alexandrov K
  TITLE     Performance benchmarking of four cell-free protein expression 
            systems.
  JOURNAL   Biotechnol Bioeng. 2015 Aug 24. doi: 10.1002/bit.25814.
  PUBMED    26301602
REFERENCE   2  (bases 1 to 1001)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Biotechnol 
            Bioeng. 2015 Aug 24. doi: 10.1002/bit.25814."
COMMENT     Sequence Label: pCellFree_G03 YY1
FEATURES             Location/Qualifiers
     source          1..1001
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    166..190
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 taccacgcgt acccaacaca cggacacatg cacgaacacg catgcagaga gatcatacat
       61 catctataga gaagtacacg taaaagtaag actcgtcgtc tgccagctac aaccgagaag
      121 gcgacgtgaa aggaacaaga aaggaggagg agggcggccg cttacaccac tttgtacaag
      181 aaagttgggc actggttgtt tttggcctta gcatgtgtta agatgtgaga tttcaggtta
      241 gttgactgag caaacttctt attacaacca tcgaaggggc acacataggg cctgtctccg
      301 gtatggattc gcacatgtgt gcgcaaattg aagtccagtg aaaagcgttt cccacagcct
      361 tcgaacgtgc actgaaaggg cttctctcca gtatgaacca gttggtgtcg ttttagtttt
      421 gaactctcaa caaaagcttt gccacattct gcacagacgt ggactctggg accgtgggtg
      481 tgcagatgtt ttctcatggc cgagttatcc ctgaacatct ttgtgcagcc tttatgaggg
      541 caagctattg ttcttggagc atcatcttct ttaatttttc ttggcttcat tctagcaaat
      601 tctgccagtt gtttgggatc tgagaggtca atgccaggta ttcctccagg aggaagtttc
      661 tttcctgtca tatattctga ataatcagga ggtgagttct ctccaatgat ctgttcttca
      721 accactgtct catggtcaat atcttttttt tcatctgagg accacatggt gaccgagaac
      781 tcgccctcca gggtcttgat ctgcacctgc ttctgctccc acttcttgtt gcccgggtcg
      841 gcgccgccgc cgcccgccgc gccggccccg ccgctgaggt aactcttctt gccgctcttc
      901 ttgccgccgc ccttcttgac gcggccgcct cccgacgacg acgagccgcc gccgccgctc
      961 ttgccggccg ccgccacggt gaccagcgtt tgttcaatgt a
//
