LOCUS       sequence-119736-        1036 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Standard for cell-free expression benchmarking..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1036)
  AUTHORS   Gagoski D, Polinkovsky ME, Mureev S, Kunert A, Johnston W, Gambin Y,
            Alexandrov K
  TITLE     Performance benchmarking of four cell-free protein expression 
            systems.
  JOURNAL   Biotechnol Bioeng. 2015 Aug 24. doi: 10.1002/bit.25814.
  PUBMED    26301602
REFERENCE   2  (bases 1 to 1036)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Biotechnol 
            Bioeng. 2015 Aug 24. doi: 10.1002/bit.25814."
COMMENT     Sequence Label: pCellFree_G03 TFDP3
FEATURES             Location/Qualifiers
     source          1..1036
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    167..191
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 taccacgcgt acccaacaca cggacacatg cacgaacacg catgcagaga gatcatacat
       61 cattctatag agaagtacac gtaaaagtaa gactcgtcgt ctgccagcta caaccgagaa
      121 ggcgacgtga aaggaacaag aaaggaggag gagggcggcc gcttacacca ctttgtacaa
      181 gaaagttggc aagtcatcct cgtcattctc actgaggtcg tcatcgttgt tgtcctcctc
      241 ctcttcctcc tcgactgctg gggtctccac cctggagcca ctgtactgag atccaccgga
      301 gcttgtggcc agcatcccaa tcgcaatgtt ggtcaggtca ctggcagaaa gccacgtgcc
      361 attagacctg gaacctgccg tcgtgaacac acctccaaaa gttccctgag ccatttctgt
      421 cacgtacggc tccagagcct ttgggaccaa atttctggcc attttaaggt cttcggcaga
      481 gcagctcccg gactctagcc caaaagtcat gcccatccac atcagcactt ctgtgtcatc
      541 gtggatttca aaggagctgt taaacttaaa cagatattct gatttgtcgt cggagatgct
      601 gcagttgatg acggtcttct tgctactgct gatgatgatg aagggcacgt ggatgactga
      661 gttgggcagc ggccgctggc tgacctgctc ctccacatac tggtttctca gcaccaggtt
      721 cttgaaagca atttgctgta gaataagttg ttgaagttca gactgtttct gctttattct
      781 ttcaagtctc ttctgtcttt ccacccgtaa gttctgacag ttctgagccg agttggtggt
      841 cagaccaatc cacttgatct tctttttctc cctggagatg atattcatgg ccatcagcac
      901 gtttaaggca tcgtaggtgc gccgttttat gtttttcacg tcataagctg actcgtttgg
      961 tgaggcgtgg ttgctggcag ctctgaactt ggcgaccagc tcgcccacca cttcctggca
     1021 ggaagtggtc cctttt
//
