LOCUS       sequence-119738-        1022 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Standard for cell-free expression benchmarking..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1022)
  AUTHORS   Gagoski D, Polinkovsky ME, Mureev S, Kunert A, Johnston W, Gambin Y,
            Alexandrov K
  TITLE     Performance benchmarking of four cell-free protein expression 
            systems.
  JOURNAL   Biotechnol Bioeng. 2015 Aug 24. doi: 10.1002/bit.25814.
  PUBMED    26301602
REFERENCE   2  (bases 1 to 1022)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Biotechnol 
            Bioeng. 2015 Aug 24. doi: 10.1002/bit.25814."
COMMENT     Sequence Label: pCellFree_G03 TFDP1
FEATURES             Location/Qualifiers
     source          1..1022
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    167..191
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 taccacgcgt acccaacaca cggacacatg cacgaacacg catgcagaga gatcatacat
       61 cattctatag agaagtacac gtaaaagtaa gactcgtcgt ctgccagcta caaccgagaa
      121 ggcgacgtga aaggaacaag aaaggaggag gagggcggcc gcttacacca ctttgtacaa
      181 gaaagttggg cagtcgtcct cgtcattctc gttgaagtca tcgtcctcct cgtcgtcctc
      241 cccgacgtag gacaccggag tctccaccct ggagccgctg tactgagacc cattggagct
      301 tgtggccagc atcccatctg caccgttggt caggtcactg gcagagaacc ttgtgccgtt
      361 agacgtggaa cctgccgtcg tgatgaacac gcctccaaca gttccctgag ccatttctgt
      421 cacgtatggc tccagagcct tggggaccag acttctggcc attttaaggt cttcggcaga
      481 gcagctcccc gactccagcc cgcaagccat gcccatccgc ttcagcactt ctatgtcatc
      541 gtggatttca aatgtgttgt caaaattaaa cagatactca aatttgtcat tggagatgct
      601 gcagtcgatg accgtcttct tgctggtgtt gacgatgatg aagggcaggt ggatgactga
      661 gttgggtggc ggtggccggc tggcctgctg ctccgcatgc cggtttctct gcaccaggtt
      721 cttgaaggca atttgctgta gaataagttc ttgaagttga gactgtttct gttttattct
      781 ttcaagtctc ctctgtcttt ccacctctaa gttctgacat tcctgagccg agttggtggg
      841 cagaccaatc cacttgatct ccttcttctc cttggagatg atgttcatgg ccattagcac
      901 gtttaaggca tcgtagacgc gccgtcttat gtttttctgg tcataagctg actcgtttgg
      961 taagatgtgg ttgtcggcag cactgaactc cgcaaccagc tcgtctgcca cttcgttgta
     1021 ag
//
