LOCUS       sequence-119741-        1064 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Standard for cell-free expression benchmarking..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1064)
  AUTHORS   Gagoski D, Polinkovsky ME, Mureev S, Kunert A, Johnston W, Gambin Y,
            Alexandrov K
  TITLE     Performance benchmarking of four cell-free protein expression 
            systems.
  JOURNAL   Biotechnol Bioeng. 2015 Aug 24. doi: 10.1002/bit.25814.
  PUBMED    26301602
REFERENCE   2  (bases 1 to 1064)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Biotechnol 
            Bioeng. 2015 Aug 24. doi: 10.1002/bit.25814."
COMMENT     Sequence Label: pCellFree_G03 CSNK2A1
FEATURES             Location/Qualifiers
     source          1..1064
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    169..193
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 taccacgcgt acccaacaca cggacacatg cacgaacacg catgcagaga gatctttaca
       61 tcatctatag agaagtacac gtaaaagtaa gactcgctcg ttctgccagc tacaaccgag
      121 aaggcgacgt gaaaggaaca agaaaggagg aggagggcgg ccgcttacac cactttgtac
      181 aagaaagttg ggtactgctg agcgccagcg gcagctggaa caggcatccc aagggggttg
      241 gcagcagcaa tcactggtga gcctgccaga ggtccaaggg gtgaaggggt tggcactgaa
      301 gaaatccctg acatcatatt ggcgctgctg acgggcgtac tgccccctgg catgctagat
      361 gaacccattc gagcctggtc cttcacaaca gtgtagaaat aggggtgctc cattgcctct
      421 cttgcagtaa gccgtgactg gtggtcatat cgcagcagtt tgtccaggaa atccaaggcc
      481 tcagggctga caaggtgctg attttcactg tggacaaagc gttcccatcg ctttcgagag
      541 tgtctgccca agatatcatt gaaacgtgga tctaattcaa tgttgtattt gtcaatatag
      601 tcatataaat cttctgtccc cagaaccttg gctatcctca ccaactgatc ataattgtca
      661 tgtccatgga aaaatggctc cttccgaaag atcatacttg ccagcataca acccaaactc
      721 cacatatcca aactataatc gtacatctga tagtctacaa gtagctcagg acctttgaag
      781 tatcgggaag caactcggac attatattct tggccaggat gataaaactc agccaaaccc
      841 cagtctatta gtcgtagctt tctgtgctca tgatcaatca tgacattatg gggcttgaca
      901 tctctgtgca taattcccat gctgtgacaa taatccaggg ccttcagaat ctcatacatg
      961 taaaatcgaa tatcatagtc tgttaacgtc tggtacaatt gcttgaagtc tgtgttgttt
     1021 acgtgttcaa aaaccaaggc gggggttcgt gacacagggt cttt
//
