LOCUS       sequence-119746-        1065 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Voltage sensing domain of Ciona with the 
            G122A/D164A/D186A/R217Q/R229I mutations fused to super ecliptic 
            pHlorin A227D.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1065)
  AUTHORS   Piao HH, Rajakumar D, Kang BE, Kim EH, Baker BJ
  TITLE     Combinatorial mutagenesis of the voltage-sensing domain enables the 
            optical resolution of action potentials firing at 60 Hz by a 
            genetically encoded fluorescent sensor of membrane potential.
  JOURNAL   J Neurosci. 2015 Jan 7;35(1):372-85. doi: 
            10.1523/JNEUROSCI.3008-14.2015.
  PUBMED    25568129
REFERENCE   2  (bases 1 to 1065)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1523/JNEUROSCI.3008-14"; journalName: "J Neurosci"; date: 
            "2015-01-7- 7"; volume: "35"; issue: "1"; pages: "372-85"
COMMENT     Sequence Label: CC3
FEATURES             Location/Qualifiers
     source          1..1065
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     61..80
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        61..79
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      99..108
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(903..931)
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
ORIGIN
        1 ctatataagc agagctctct ggctaactag agaacccact gcttactggc ttatcgaaat
       61 taatacgact cactataggg agacccaagc tggctagcgc caccatggag ggattcgacg
      121 gttcagattt tagtcctcca gctgatttag ttggcgttga cggtgcagtc atgcggaacg
      181 tcgttgacgt cacgataaat ggtgacgtca ctgctccgcc gaaagctgcg ccaagaaaaa
      241 gtgaatcggt aaagaaagtt cattggaatg atgtagacca aggaccgagt gaaaaaccag
      301 agacaagaca ggaggaacga atagatatac ccgagatttc aggtctatgg tggggcgaga
      361 atgaacatgg agtggacgat gggagaatgg agatacctac tactggtgta ggtcgcgtcc
      421 agtttcggat ccgagcagtg attgatcatc tagggatgcg agtctttgcc gtcttcctaa
      481 ttttcttgga catcatcctc atgatcattg acctcagtct tatcgataaa agtgaatctt
      541 cacaatcctt ttatgacggg atggccttgg ctctttcttg ttatttcatg ctggctttag
      601 gattaaggat atttgcctac gggcccaagc ttttcttcac caacccttgg gaggtggctg
      661 ccggtttgat tatcgttgtc acattcgtcg tcacgatatt ttacactgtg ttagatgaat
      721 acggtaccga aacaggagcc gatggtttgg ggcagttggt tgtgttggcc agattgctgc
      781 gtgtggttat cttagcaaga atattttatt cccaccaaca aatgaaggct tcaagcagaa
      841 gaacaatatc acagaacaaa agaagatatc gcatgagtaa aggagaagaa cttttcactg
      901 gagttgtccc aattcttgtt gaattagatg gtgatgttaa tgggcacaaa ttttctgtca
      961 gtggagaggg tgaaggtgat gcaacatacg gaaaacttac ccttaaattt atttgcacta
     1021 ctggaaaact acctgttcca tggccaacac ttgtcactac tttaa
//
