LOCUS       sequence-119755-        1030 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Voltage sensing domain of Ciona with the A154D/R217Q/R229I mutations
            fused to super ecliptic pHlorin A227D.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1030)
  AUTHORS   Piao HH, Rajakumar D, Kang BE, Kim EH, Baker BJ
  TITLE     Combinatorial mutagenesis of the voltage-sensing domain enables the 
            optical resolution of action potentials firing at 60 Hz by a 
            genetically encoded fluorescent sensor of membrane potential.
  JOURNAL   J Neurosci. 2015 Jan 7;35(1):372-85. doi: 
            10.1523/JNEUROSCI.3008-14.2015.
  PUBMED    25568129
REFERENCE   2  (bases 1 to 1030)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1523/JNEUROSCI.3008-14"; journalName: "J Neurosci"; date: 
            "2015-01-7- 7"; volume: "35"; issue: "1"; pages: "372-85"
COMMENT     Sequence Label: Triple mutant
FEATURES             Location/Qualifiers
     source          1..1030
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     69..86
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     CDS             complement(127..843)
                     /codon_start=1
                     /product="pH-sensitive mutant of green fluorescent protein 
                     (Ng et al., 2002)"
                     /label=superecliptic pHluorin
                     /note="excited strongly by blue light at neutral pH but 
                     minimally fluorescent at acidic pH"
                     /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNDHQVYIMADKQKNGIKA
                     NFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLFTTSTLSKDPNEKRDHMVLLE
                     FVTADGITHGMDELYK"
     primer_bind     complement(170..190)
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
     primer_bind     785..813
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
ORIGIN
        1 agtgggagtg gcaccttcca gggtcaagga aggcacgggg gaggggcaaa caacagatgg
       61 ctggcaacta gaaggcacag tcgaggctga tcagcgggtt taaacgggcc ctctagactc
      121 gagtcatcat ttgtatagtt catccatgcc atgtgtaatc ccatcagctg ttacaaactc
      181 aagaaggacc atgtggtctc tcttttcgtt gggatctttc gaaagagtag aagttgtaaa
      241 caggtaatgg ttgtctggta aaaggacggg cccatcgcca attggagtat tttgttgata
      301 atggtctgct agttgaacgc ctccatcttc aatgttgtgt ctaattttga agttagcttt
      361 gattccattc ttttgtttgt ctgccatgat gtacacctgg tgatcgttat agttgtattc
      421 caatttgtgt ccaagaatgt ttccatcttc tttaaaatca atacctttta actcgattct
      481 attaacaagg gtatcacctt caaacttgac ttcagcacgt gtcttgtagt tcccgtcatc
      541 tttgaaaaat atagttcttt cctgtacata accttcgggc atggcactct tgaaaaagtc
      601 atgccgtttc atatgatctg ggtatcttga aaagcattga acaccataag ttaaagtagt
      661 gacaagtgtt ggccatggaa caggtagttt tccagtagtg caaataaatt taagggtaag
      721 ttttccgtat gttgcatcac cttcaccctc tccactgaca gaaaatttgt gcccattaac
      781 atcaccatct aattcaacaa gaattgggac aactccagtg aaaagttctt ctcctttact
      841 catgcgatat cttcttttgt tctgtgatat tgttcttctg cttgaagcct tcatttgttg
      901 gtgggaataa aatattcttg ctaagataac cacacgcagc aatctggcca acacaaccaa
      961 ctgccccaaa ccatcggctc ctgtttcggt accgtattca tctaacacag tgtaaaatat
     1021 cgtgacgacg
//
