LOCUS       sequence-119756-        1061 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Voltage sensing domain of Ciona with the A154D/R217Q/R229I mutations
            fused to super ecliptic pHlorin A227D.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1061)
  AUTHORS   Piao HH, Rajakumar D, Kang BE, Kim EH, Baker BJ
  TITLE     Combinatorial mutagenesis of the voltage-sensing domain enables the 
            optical resolution of action potentials firing at 60 Hz by a 
            genetically encoded fluorescent sensor of membrane potential.
  JOURNAL   J Neurosci. 2015 Jan 7;35(1):372-85. doi: 
            10.1523/JNEUROSCI.3008-14.2015.
  PUBMED    25568129
REFERENCE   2  (bases 1 to 1061)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1523/JNEUROSCI.3008-14"; journalName: "J Neurosci"; date: 
            "2015-01-7- 7"; volume: "35"; issue: "1"; pages: "372-85"
COMMENT     Sequence Label: Triple mutant
FEATURES             Location/Qualifiers
     source          1..1061
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      823..832
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(851..870)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(852..870)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        complement(915..1014)
                     /label=CMVd1 promoter
                     /note="truncated human cytomegalovirus (CMV) immediate 
                     early promoter that yields slightly reduced expression 
                     relative to the full-length promoter"
     primer_bind     complement(944..964)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
ORIGIN
        1 catctaattc aacaagaatt gggacaactc cagtgaaaag ttcttctcct ttactcatgc
       61 gatatcttct tttgttctgt gatattgttc ttctgcttga agccttcatt tgttggtggg
      121 aataaaatat tcttgctaag ataaccacac gcagcaatct ggccaacaca accaactgcc
      181 ccaaaccatc ggctcctgtt tcggtaccgt attcatctaa cacagtgtaa aatatcgtga
      241 cgacgaatgt gacaacgata atcaaaccat cagccacctc ccaagggttg gtgaagaaaa
      301 gcttgggccc gtaggcaaat atccttaatc ctaaatccag catgaaataa caagaaagag
      361 ccaagtccat cccgtcataa aaggattgtg aagattcact tttatcgata agactgagat
      421 caatgatcat gaggatgatg tccaagaaaa ttaggaagac tccaaagact cgcatcccta
      481 gatgatcaat cactgctcgg atccgaaact ggacgcgacc tacaccagta gtaggtatct
      541 ccattctccc atcgtccact ccatgttcat tctcgcccca ccatagacct gaaatctcgg
      601 gtatatctat tcgttcctcc tgtcttgtct ctggtttttc actcggtcct tggtctacat
      661 cattccaatg aactttcttt accgattcac tttttcttgg cgcagctttc ggcggagcag
      721 tgacgtcacc atttatcgtg acgtcaacga cgttccgcat gactgcaccg tcaacgccaa
      781 ctaaatcagc tggaggacta aaatctgaac cgtcgaatcc ctccatggtg gcgctagcca
      841 gcttgggtct ccctatagtg agtcgtatta atttcgataa gccagtaagc agtgggttct
      901 ctagttagcc agagagctct gcttatatag acctcccacc gtacacgcct accgcccatt
      961 tgcgtcaatg gggcggagtt gttacgacat tttggaaagt cccgttgatt ttggtgccaa
     1021 aacaaactcc cattgacgtc aatggggtgg agacttggaa t
//
