LOCUS       sequence-119757-         745 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Voltage sensing domain of Ciona with the A154D/R217Q/R229I mutations
            fused to super ecliptic pHlorin A227D.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 745)
  AUTHORS   Piao HH, Rajakumar D, Kang BE, Kim EH, Baker BJ
  TITLE     Combinatorial mutagenesis of the voltage-sensing domain enables the 
            optical resolution of action potentials firing at 60 Hz by a 
            genetically encoded fluorescent sensor of membrane potential.
  JOURNAL   J Neurosci. 2015 Jan 7;35(1):372-85. doi: 
            10.1523/JNEUROSCI.3008-14.2015.
  PUBMED    25568129
REFERENCE   2  (bases 1 to 745)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1523/JNEUROSCI.3008-14"; journalName: "J Neurosci"; date: 
            "2015-01-7- 7"; volume: "35"; issue: "1"; pages: "372-85"
COMMENT     Sequence Label: Triple mutant
FEATURES             Location/Qualifiers
     source          1..745
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     61..80
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        61..79
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      99..108
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 ctatataagc agagctctct ggctaactag agaacccact gcttactggc ttatcgaaat
       61 taatacgact cactataggg agacccaagc tggctagcgc caccatggag ggattcgacg
      121 gttcagattt tagtcctcca gctgatttag ttggcgttga cggtgcagtc atgcggaacg
      181 tcgttgacgt cacgataaat ggtgacgtca ctgctccgcc gaaagctgcg ccaagaaaaa
      241 gtgaatcggt aaagaaagtt cattggaatg atgtagacca aggaccgagt gaaaaaccag
      301 agacaagaca ggaggaacga atagatatac ccgagatttc aggtctatgg tggggcgaga
      361 atgaacatgg agtggacgat gggagaatgg agatacctac tactggtgta ggtcgcgtcc
      421 agtttcggat ccgagcagtg attgatcatc tagggatgcg agtctttgga gtcttcctaa
      481 ttttcttgga catcatcctc atgatcattg atctcagtct tatcgataaa agtgaatctt
      541 cacaatcctt ttatgacggg atggacttgg ctctttcttg ttatttcatg ctggatttag
      601 gattaaggat atttgcctac gggcccaagc ttttcttcac caacccttgg gaggtggctg
      661 atggtttgat tatcgttgtc acattcgtcg tcacgatatt ttacactgtg ttagatgaat
      721 acggtaccga aacaggagcc gatgg
//
