LOCUS       sequence-119782-        1056 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Consists of the triple mutant voltage sensing domain and the 
            optimized linker between the S4 transmembrane domain and the super 
            ecliptic pHlorin A227D.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1056)
  AUTHORS   Piao HH, Rajakumar D, Kang BE, Kim EH, Baker BJ
  TITLE     Combinatorial mutagenesis of the voltage-sensing domain enables the 
            optical resolution of action potentials firing at 60 Hz by a 
            genetically encoded fluorescent sensor of membrane potential.
  JOURNAL   J Neurosci. 2015 Jan 7;35(1):372-85. doi: 
            10.1523/JNEUROSCI.3008-14.2015.
  PUBMED    25568129
REFERENCE   2  (bases 1 to 1056)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1523/JNEUROSCI.3008-14"; journalName: "J Neurosci"; date: 
            "2015-01-7- 7"; volume: "35"; issue: "1"; pages: "372-85"
COMMENT     Sequence Label: Bongwoori
FEATURES             Location/Qualifiers
     source          1..1056
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     68..85
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     CDS             complement(130..846)
                     /codon_start=1
                     /product="pH-sensitive mutant of green fluorescent protein 
                     (Ng et al., 2002)"
                     /label=superecliptic pHluorin
                     /note="excited strongly by blue light at neutral pH but 
                     minimally fluorescent at acidic pH"
                     /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNDHQVYIMADKQKNGIKA
                     NFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLFTTSTLSKDPNEKRDHMVLLE
                     FVTADGITHGMDELYK"
     primer_bind     complement(173..193)
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
     primer_bind     788..816
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
ORIGIN
        1 gtgggagtgg caccttccag ggtcaaggaa ggcacggggg aggggcaaac aacagatggc
       61 tggcaactag aaggcacagt cgaggctgat cagcgggttt aaacgggccc tctagactcg
      121 agttctagat catttgtata gttcatccat gccatgtgta atcccatcag ctgttacaaa
      181 ctcaagaagg accatgtggt ctctcttttc gttgggatct ttcgaaagag tagaagttgt
      241 aaacaggtaa tggttgtctg gtaaaaggac gggcccatcg ccaattggag tattttgttg
      301 ataatggtct gctagttgaa cgcctccatc ttcaatgttg tgtctaattt tgaagttagc
      361 tttgattcca ttcttttgtt tgtctgccat gatgtacacc tggtgatcgt tatagttgta
      421 ttccaatttg tgtccaagaa tgtttccatc ttctttaaaa tcaatacctt ttaactcgat
      481 tctattaaca agggtatcac cttcaaactt gacttcagca cgtgtcttgt agttcccgtc
      541 atctttgaaa aatatagttc tttcctgtac ataaccttcg ggcatggcac tcttgaaaaa
      601 gtcatgccgt ttcatatgat ctgggtatct tgaaaagcat tgaacaccat aagttaaagt
      661 agtgacaagt gttggccatg gaacaggtag ttttccagta gtgcaaataa atttaagggt
      721 aagttttccg tatgttgcat caccttcacc ctctccactg acagaaaatt tgtgcccatt
      781 aacatcacca tctaattcaa caagaattgg gacaactcca gtgaaaagtt cttctccttt
      841 actcatggga tccccttgtt ggtgggaata aaatattctt gctaagataa ccacacgcag
      901 caatctggcc aacacaacca actgccccaa accatcggct cctgtttcgg taccgtattc
      961 atctaacaca gtgtaaaata tcgtgacgac gaatgtgaca acgataatca aaccatcagc
     1021 cacctcccaa gggttggtga agaaaagctt gggccc
//
