LOCUS       sequence-119783-         863 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 863)
  AUTHORS   Grashoff C, Hoffman BD, Brenner MD, Zhou R, Parsons M, Yang MT, 
            McLean MA, Sligar SG, Chen CS, Ha T, Schwartz MA
  TITLE     Measuring mechanical tension across vinculin reveals regulation of 
            focal adhesion dynamics.
  JOURNAL   Nature. 2010 Jul 8. 466(7303):263-6.
  PUBMED    20613844
REFERENCE   2  (bases 1 to 863)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nature. 
            2010 Jul 8. 466(7303):263-6."
COMMENT     Sequence Label: Tailless VinculinTS
FEATURES             Location/Qualifiers
     source          1..863
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(139..846)
                     /codon_start=1
                     /product="monomeric teal (cyan) fluorescent protein (Ai et 
                     al., 2006)"
                     /label=mTFP1
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEETTMGVIKPDMKIKLKMEGNVNGHAFVIEGEGEGKPYDG
                     TNTINLEVKEGAPLPFSYDILTTAFAYGNRAFTKYPDDIPNYFKQSFPEGYSWERTMTF
                     EDKGIVKVKSDISMEEDSFIYEIHLKGENFPPNGPVMQKKTTGWDASTERMYVRDGVLK
                     GDVKHKLLLEGGGHHRVDFKTIYRAKKAVKLPDYHFVDHRIEILNHDKDYNKVTVYESA
                     VARNSTDGMDELYK"
ORIGIN
        1 ctcgcccttg ctcaccatag caccacctgg acctgcacca cctggacctg caccacctgg
       61 acctgcacca ccgggccctg caccacctgg ccctgcacca cctggccctg caccacctgg
      121 ccctgcacca cctggcccct tgtacagctc gtccatgccg tcggtggagt tgcgggccac
      181 ggcgctctcg taaacggtca ccttgttgta gtccttgtcg tggttcagga tctcgatgcg
      241 gtggtccaca aagtgatagt cgggcagctt caccgccttc ttggccctgt agatggtctt
      301 gaagtcaacg cggtggtggc cgccgccctc cagcagcagc ttgtgcttga cgtcgccctt
      361 cagcacgccg tcgcgcacgt acatcctctc ggtggaggcg tcccagccgg tggtcttctt
      421 ctgcatcacg gggccgttgg gggggaagtt ctcgcccttg aggtgtatct cgtagatgaa
      481 ggagtcctcc tccatggaga tgtcggactt caccttcacg atgcccttgt cctcgaaggt
      541 catggtgcgc tcccaagagt agccctcggg gaaggactgc ttgaagtagt tggggatgtc
      601 gtcggggtac ttggtgaagg ccctgttgcc gtaggcgaac gcggtggtca gaatgtcgta
      661 ggagaagggc aggggggctc cctccttcac ctccaggttg atggtgttgg tgccgtcgta
      721 gggcttgccc tcgccctcgc cctcgatcac gaaggcgtgg ccattcacgt tgccctccat
      781 cttcagcttg atcttcatgt cgggcttgat tacgcccatt gtggtctcct cgcccttgct
      841 caccatctcg acctcatctt ttt
//
