LOCUS       sequence-119785-         966 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  To fuse your protein of interest to the C-terminus of BirA(R118G) 
            and use in proximity-dependent biotin identification (BioID); myc 
            tag.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 966)
  AUTHORS   Roux KJ, Kim DI, Raida M, Burke B
  TITLE     A promiscuous biotin ligase fusion protein identifies proximal and 
            interacting proteins in mammalian cells.
  JOURNAL   J Cell Biol. 2012 Mar 12.
  PUBMED    22412018
REFERENCE   2  (bases 1 to 966)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Cell 
            Biol. 2012 Mar 12."
COMMENT     Sequence Label: pcDNA3.1 mycBioID
FEATURES             Location/Qualifiers
     source          1..966
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     47..66
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        47..65
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      83..92
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             92..121
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
ORIGIN
        1 ctctctggct aactagagaa cccactgctt actggcttat cgaaattaat acgactcact
       61 atagggagac ccaagctggc tagccaccat ggaacaaaaa ctcatctcag aagaggatct
      121 cgacaaggac aacaccgtgc ccctgaagct gatcgccctg ctggccaacg gcgagttcca
      181 ctctggcgag cagctgggag agaccctggg aatgagcaga gccgccatca acaagcacat
      241 ccagacactg agagactggg gagtggacgt gttcaccgtg cctggcaagg gctacagcct
      301 gcctgagcct atccagctgc tgaacgccaa gcagatcctg ggacagctgg atggcggaag
      361 cgtggccgtg ctgcctgtga tcgactccac caatcagtac ctgctggaca gaatcggaga
      421 gctgaagtcc ggcgacgcct gcatcgccga gtaccagcag gctggcagag gaggcagagg
      481 acggaagtgg ttcagcccat tcggagccaa cctgtacctg tccatgttct ggagactgga
      541 gcagggacct gctgctgcca tcggactgag tctggtgatc ggaatcgtga tggccgaggt
      601 gctgagaaag ctgggagccg acaaggtgag agtgaagtgg cctaatgacc tgtacctcca
      661 ggaccgcaag ctggctggca tcctggtgga gctgacaggc aagacaggcg atgccgctca
      721 gatcgtgatc ggagccggaa tcaacatggc catgagaaga gtggaggaga gcgtggtgaa
      781 ccagggctgg atcaccctgc aggaggctgg catcaacctg gaccggaaca ccctggccgc
      841 catgctgatc agagagctga gagccgctct ggagctgttc gagcaggagg gactggctcc
      901 ttacctgagc agatgggaga agctggacaa cttcatcaac agacctgtga agctgatcat
      961 cggcga
//
