LOCUS       sequence-119792-        1090 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Consists of the triple mutant voltage sensing domain and the 
            optimized linker between the S4 transmembrane domain and the super 
            ecliptic pHlorin A227D.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1090)
  AUTHORS   Piao HH, Rajakumar D, Kang BE, Kim EH, Baker BJ
  TITLE     Combinatorial mutagenesis of the voltage-sensing domain enables the 
            optical resolution of action potentials firing at 60 Hz by a 
            genetically encoded fluorescent sensor of membrane potential.
  JOURNAL   J Neurosci. 2015 Jan 7;35(1):372-85. doi: 
            10.1523/JNEUROSCI.3008-14.2015.
  PUBMED    25568129
REFERENCE   2  (bases 1 to 1090)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1523/JNEUROSCI.3008-14"; journalName: "J Neurosci"; date: 
            "2015-01-7- 7"; volume: "35"; issue: "1"; pages: "372-85"
COMMENT     Sequence Label: Bongwoori
FEATURES             Location/Qualifiers
     source          1..1090
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(804..823)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(805..823)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        complement(868..1071)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(897..917)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
ORIGIN
        1 tctaattcaa caagaattgg gacaactcca gtgaaaagtt cttctccttt actcatggga
       61 tccccttgtt ggtgggaata aaatattctt gctaagataa ccacacgcag caatctggcc
      121 aacacaacca actgccccaa accatcggct cctgtttcgg taccgtattc atctaacaca
      181 gtgtaaaata tcgtgacgac gaatgtgaca acgataatca aaccatcagc cacctcccaa
      241 gggttggtga agaaaagctt gggcccgtag gcaaatatcc ttaatcctaa atccagcatg
      301 aaataacaag aaagagccaa gtccatcccg tcataaaagg attgtgaaga ttcactttta
      361 tcgataagac tgagatcaat gatcatgagg atgatgtcca agaaaattag gaagactcca
      421 aagactcgca tccctagatg atcaatcact gctcggatcc gaaactggac gcgacctaca
      481 ccagtagtag gtatctccat tctcccatcg tccactccat gttcattctc gccccaccat
      541 agacctgaaa tctcgggtat atctattcgt tcctcctgtc ttgtctctgg tttttcactc
      601 ggtccttggt ctacatcatt ccaatgaact ttctttaccg attcactttt tcttggcgca
      661 gctttcggcg gagcagtgac gtcaccattt atcgtgacgt caacgacgtt ccgcatgact
      721 gcaccgtcaa cgccaactaa atcagctgga ggactaaaat ctgaaccgtc gaatccctcc
      781 atggtgctag ccagcttggg tctccctata gtgagtcgta ttaatttcga taagccagta
      841 agcagtgggt tctctagtta gccagagagc tctgcttata tagacctccc accgtacacg
      901 cctaccgccc atttgcgtca atggggcgga gttgttacga cattttggaa agtcccgttg
      961 attttggtgc caaaacaaac tcccattgac gtcaatgggg tggagacttg gaaatccccg
     1021 tgagtcaaac cgctatccac gcccattgat gtactgccaa aaccgcatca ccatggtaat
     1081 agcgatgact
//
