LOCUS       sequence-119795-         740 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Consists of the triple mutant voltage sensing domain and the 
            optimized linker between the S4 transmembrane domain and the super 
            ecliptic pHlorin A227D.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 740)
  AUTHORS   Piao HH, Rajakumar D, Kang BE, Kim EH, Baker BJ
  TITLE     Combinatorial mutagenesis of the voltage-sensing domain enables the 
            optical resolution of action potentials firing at 60 Hz by a 
            genetically encoded fluorescent sensor of membrane potential.
  JOURNAL   J Neurosci. 2015 Jan 7;35(1):372-85. doi: 
            10.1523/JNEUROSCI.3008-14.2015.
  PUBMED    25568129
REFERENCE   2  (bases 1 to 740)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1523/JNEUROSCI.3008-14"; journalName: "J Neurosci"; date: 
            "2015-01-7- 7"; volume: "35"; issue: "1"; pages: "372-85"
COMMENT     Sequence Label: Bongwoori
FEATURES             Location/Qualifiers
     source          1..740
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     61..80
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        61..79
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 ctatataagc agagctctct ggctaactag agaacccact gcttactggc ttatcgaaat
       61 taatacgact cactataggg agacccaagc tggctagcac catggaggga ttcgacggtt
      121 cagattttag tcctccagct gatttagttg gcgttgacgg tgcagtcatg cggaacgtcg
      181 ttgacgtcac gataaatggt gacgtcactg ctccgccgaa agctgcgcca agaaaaagtg
      241 aatcggtaaa gaaagttcat tggaatgatg tagaccaagg accgagtgaa aaaccagaga
      301 caagacagga ggaacgaata gatatacccg agatttcagg tctatggtgg ggcgagaatg
      361 aacatggagt ggacgatggg agaatggaga tacctactac tggtgtaggt cgcgtccagt
      421 ttcggatccg agcagtgatt gatcatctag ggatgcgagt ctttggagtc ttcctaattt
      481 tcttggacat catcctcatg atcattgatc tcagtcttat cgataaaagt gaatcttcac
      541 aatcctttta tgacgggatg gacttggctc tttcttgtta tttcatgctg gatttaggat
      601 taaggatatt tgcctacggg cccaagcttt tcttcaccaa cccttgggag gtggctgatg
      661 gtttgattat cgttgtcaca ttcgtcgtca cgatatttta cactgtgtta gatgaatacg
      721 gtaccgaaac aggagccgat
//
