LOCUS       sequence-119799-        1447 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Consists of the triple mutant voltage sensing domain and the 
            optimized linker between the S4 transmembrane domain and the super 
            ecliptic pHlorin A227D.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1447)
  AUTHORS   Piao HH, Rajakumar D, Kang BE, Kim EH, Baker BJ
  TITLE     Combinatorial mutagenesis of the voltage-sensing domain enables the 
            optical resolution of action potentials firing at 60 Hz by a 
            genetically encoded fluorescent sensor of membrane potential.
  JOURNAL   J Neurosci. 2015 Jan 7;35(1):372-85. doi: 
            10.1523/JNEUROSCI.3008-14.2015.
  PUBMED    25568129
REFERENCE   2  (bases 1 to 1447)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1523/JNEUROSCI.3008-14"; journalName: "J Neurosci"; date: 
            "2015-01-7- 7"; volume: "35"; issue: "1"; pages: "372-85"
COMMENT     Sequence Label: Bongwoori
FEATURES             Location/Qualifiers
     source          1..1447
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             731..1447
                     /codon_start=1
                     /product="pH-sensitive mutant of green fluorescent protein 
                     (Ng et al., 2002)"
                     /label=superecliptic pHluorin
                     /note="excited strongly by blue light at neutral pH but 
                     minimally fluorescent at acidic pH"
                     /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNDHQVYIMADKQKNGIKA
                     NFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLFTTSTLSKDPNEKRDHMVLLE
                     FVTADGITHGMDELYK"
     primer_bind     complement(761..789)
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
     primer_bind     1384..1404
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
ORIGIN
        1 caccatggag ggattcgacg gttcagattt tagtcctcca gctgatttag ttggcgttga
       61 cggtgcagtc atgcggaacg tcgttgacgt cacgataaat ggtgacgtca ctgctccgcc
      121 gaaagctgcg ccaagaaaaa gtgaatcggt aaagaaagtt cattggaatg atgtagacca
      181 aggaccgagt gaaaaaccag agacaagaca ggaggaacga atagatatac ccgagatttc
      241 aggtctatgg tggggcgaga atgaacatgg agtggacgat gggagaatgg agatacctac
      301 tactggtgta ggtcgcgtcc agtttcggat ccgagcagtg attgatcatc tagggatgcg
      361 agtctttgga gtcttcctaa ttttcttgga catcatcctc atgatcattg atctcagtct
      421 tatcgataaa agtgaatctt cacaatcctt ttatgacggg atggacttgg ctctttcttg
      481 ttatttcatg ctggatttag gattaaggat atttgcctac gggcccaagc ttttcttcac
      541 caacccttgg gaggtggctg atggtttgat tatcgttgtc acattcgtcg tcacgatatt
      601 ttacactgtg ttagatgaat acggtaccga aacaggagcc gatggtttgg ggcagttggt
      661 tgtgttggcc cgtttgctgc gtgtggttat cttagcaaga atattttatt cccaccaaca
      721 aggggatccc atgagtaaag gagaagaact tttcactgga gttgtcccaa ttcttgttga
      781 attagatggt gatgttaatg ggcacaaatt ttctgtcagt ggagagggtg aaggtgatgc
      841 aacatacgga aaacttaccc ttaaatttat ttgcactact ggaaaactac ctgttccatg
      901 gccaacactt gtcactactt taacttatgg tgttcaatgc ttttcaagat acccagatca
      961 tatgaaacgg catgactttt tcaagagtgc catgcccgaa ggttatgtac aggaaagaac
     1021 tatatttttc aaagatgacg ggaactacaa gacacgtgct gaagtcaagt ttgaaggtga
     1081 tacccttgtt aatagaatcg agttaaaagg tattgatttt aaagaagatg gaaacattct
     1141 tggacacaaa ttggaataca actataacga tcaccaggtg tacatcatgg cagacaaaca
     1201 aaagaatgga atcaaagcta acttcaaaat tagacacaac attgaagatg gaggcgttca
     1261 actagcagac cattatcaac aaaatactcc aattggcgat gggcccgtcc ttttaccaga
     1321 caaccattac ctgtttacaa cttctactct ttcgaaagat cccaacgaaa agagagacca
     1381 catggtcctt cttgagtttg taacagctga tgggattaca catggcatgg atgaactata
     1441 caaatga
//
