LOCUS       sequence-119801-         770 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Cytosolic FRET, Excitation: , Emission: .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 770)
  AUTHORS   Davidson Lab
  TITLE     FRET Indicators - Live and Fixed Cells
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 770)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: mTagBFP2-mEmerald-FRET
FEATURES             Location/Qualifiers
     source          1..770
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             59..769
                     /codon_start=1
                     /product="enhanced monomeric blue fluorescent protein 
                     (Subach et al., 2011)"
                     /label=mTagBFP2
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELIKENMHMKLYMEGTVDNHHFKCTSEGEGKPYEGTQTM
                     RIKVVEGGPLPFAFDILATSFLYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGG
                     VLTATQDTSLQDGCLIYNVKIRGVNFTSNGPVMQKKTLGWEAFTETLYPADGGLEGRND
                     MALKLVGGSHLIANAKTTYRSKKPAKNLKMPGVYYVDYRLERIKEANNETYVEQHEVAV
                     ARYCDLPSKLGHKLN"
ORIGIN
        1 ccgtcagatc cgctagcgct accggactca gatctcgagc tcaagcttcg aattcaccat
       61 ggtgtctaag ggcgaagagc tgattaagga gaacatgcac atgaagctgt acatggaggg
      121 caccgtggac aaccatcact tcaagtgcac atccgagggc gaaggcaagc cctacgaggg
      181 cacccagacc atgagaatca aggtggtcga gggcggccct ctccccttcg ccttcgacat
      241 cctggctact agcttcctct acggcagcaa gaccttcatc aaccacaccc agggcatccc
      301 cgacttcttc aagcagtcct tccctgaggg cttcacatgg gagagagtca ccacatacga
      361 agacgggggc gtgctgaccg ctacccagga caccagcctc caggacggct gcctcatcta
      421 caacgtcaag atcagagggg tgaacttcac atccaacggc cctgtgatgc agaagaaaac
      481 actcggctgg gaggccttca ccgagacgct gtaccccgct gacggcggcc tggaaggcag
      541 aaacgacatg gccctgaagc tcgtgggcgg gagccatctg atcgcaaacg ccaagaccac
      601 atatagatcc aagaaacccg ctaagaacct caagatgcct ggcgtctact atgtggacta
      661 cagactggaa agaatcaagg aggccaacaa cgagacctac gtcgagcagc acgaggtggc
      721 agtggccaga tactgcgacc tccctagcaa actggggcac aagcttaatt
//
