LOCUS       sequence-119805-         940 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Standard for cell-free expression benchmarking..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 940)
  AUTHORS   Gagoski D, Polinkovsky ME, Mureev S, Kunert A, Johnston W, Gambin Y,
            Alexandrov K
  TITLE     Performance benchmarking of four cell-free protein expression 
            systems.
  JOURNAL   Biotechnol Bioeng. 2015 Aug 24. doi: 10.1002/bit.25814.
  PUBMED    26301602
REFERENCE   2  (bases 1 to 940)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Biotechnol 
            Bioeng. 2015 Aug 24. doi: 10.1002/bit.25814."
COMMENT     Sequence Label: pCellFree_G03 S100A1
FEATURES             Location/Qualifiers
     source          1..940
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    168..192
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(475..499)
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             complement(500..523)
                     /codon_start=1
                     /product="recognition and cleavage site for human 
                     rhinovirus 3C and PreScission proteases"
                     /label=HRV 3C site
                     /translation="LEVLFQGP"
     primer_bind     complement(566..587)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     915..934
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
ORIGIN
        1 ggtaccacgc gtacccaaca cacggacaca tgcacgaaca cgcatgcaga gagatcatac
       61 atcatctata gagaagtaca cgtaaaagta agactcgtcg tctgccagct acaaccgaga
      121 aggcgacgtg aaaggaacaa gaaaggagga ggagggcggc cgcttacacc actttgtaca
      181 agaaagttgg gcaactgttc tcccagaaga aattgttaca ggccactgtg agagcagcca
      241 caagcaccac atactcctgg aagtccacct ccccgtctcc attctcgtct agctccttca
      301 tcaccttgtc cacagcatcc acatccttct gggcatccag gaagccagag agctccgtct
      361 gcagcagctc tttcagctcc ttcttgctca gcttgtactt gtccccctct ttgcccgagt
      421 gggcgtggaa cacgttgatg agggtctcca tcgccgtctc cagctcagag cccatgccaa
      481 cttttttgta caaacttgtc ggaccctgga agagcacctc cagcttgtat agctcgtcca
      541 tgccgagagt gatcccggcg gcggtcacga actccagcag gaccatgtga tcgcgcttct
      601 cgttggggtc tttgctcagg gcggactggg tgctcaggta gtggttgtcg ggcagcagca
      661 cggggccgtc gccgatgggg gtgttctgct ggtagtggtc ggcgagctgc acgctgccgt
      721 cctcgatgtt gtggcggatc ttgaagttca ccttgatgcc gttcttctgc ttgtcggcca
      781 tgatatagac gttgtggctg ttgtagttgt actccagctt gtgccccagg atgttgccgt
      841 cctccttgaa gtcgatgccc ttcagctcga tgcggttcac cagggtgtcg ccctcgaact
      901 tcacctcggc gcgggtcttg tagttgccgt cgtccttgaa
//
