LOCUS       sequence-119816-         951 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Standard for cell-free expression benchmarking..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 951)
  AUTHORS   Gagoski D, Polinkovsky ME, Mureev S, Kunert A, Johnston W, Gambin Y,
            Alexandrov K
  TITLE     Performance benchmarking of four cell-free protein expression 
            systems.
  JOURNAL   Biotechnol Bioeng. 2015 Aug 24. doi: 10.1002/bit.25814.
  PUBMED    26301602
REFERENCE   2  (bases 1 to 951)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Biotechnol 
            Bioeng. 2015 Aug 24. doi: 10.1002/bit.25814."
COMMENT     Sequence Label: pCellFree_G03 TCEB2
FEATURES             Location/Qualifiers
     source          1..951
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    169..193
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(548..572)
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             complement(573..596)
                     /codon_start=1
                     /product="recognition and cleavage site for human 
                     rhinovirus 3C and PreScission proteases"
                     /label=HRV 3C site
                     /translation="LEVLFQGP"
     primer_bind     complement(639..660)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 gggtaccacg cgtacccaac acacggacac atgcacgaac acgcatgcag agagatcata
       61 catcatctat agagaagtac acgtaaaagt aagactcgtc gtctgccagc tacaaccgag
      121 aaggcgacgt gaaaggaaca agaaaggagg aggagggcgg ccgcttacac cactttgtac
      181 aagaaagttg ggcactgcac ggctttttca ttggcactgc ttcccgagtc ctggggcttc
      241 atcacatcgg gcagctctgg cgggctggaa aacggctcga tgcacagggc ctcaaaggtg
      301 tcatctgccc ggaaggccag ccccactgtg gctggggcct gtggccgtgc tgtttgactg
      361 gtgaagccac actcgcccag tgtcttgcca tcatccaaga gttggtcatc cttgtacagc
      421 cgctgctcgt caggaggccg cttgaggatg ccctcgacga tgcgcttcag ttcgaacacc
      481 gtgctggact ccttggcgtc cgtgaagatg gtggtcttgt ggcgccggat cctgaggaac
      541 acgtccatgc caactttttt gtacaaactt gtcggaccct ggaagagcac ctccagcttg
      601 tatagctcgt ccatgccgag agtgatcccg gcggcggtca cgaactccag caggaccatg
      661 tgatcgcgct tctcgttggg gtctttgctc agggcggact gggtgctcag gtagtggttg
      721 tcgggcagca gcacggggcc gtcgccgatg ggggtgttct gctggtagtg gtcggcgagc
      781 tgcacgctgc cgtcctcgat gttgtggcgg atcttgaagt tcaccttgat gccgttcttc
      841 tgcttgtcgg ccatgatata gacgttgtgg ctgttgtagt tgtactccag cttgtgcccc
      901 aggatgttgc cgtcctcctt gaagtcgatg cccttcagct cgatgcggtt c
//
