LOCUS       sequence-119818-         928 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Standard for cell-free expression benchmarking..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 928)
  AUTHORS   Gagoski D, Polinkovsky ME, Mureev S, Kunert A, Johnston W, Gambin Y,
            Alexandrov K
  TITLE     Performance benchmarking of four cell-free protein expression 
            systems.
  JOURNAL   Biotechnol Bioeng. 2015 Aug 24. doi: 10.1002/bit.25814.
  PUBMED    26301602
REFERENCE   2  (bases 1 to 928)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Biotechnol 
            Bioeng. 2015 Aug 24. doi: 10.1002/bit.25814."
COMMENT     Sequence Label: pCellFree_G03 BATF3
FEATURES             Location/Qualifiers
     source          1..928
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    168..192
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(577..601)
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             complement(602..625)
                     /codon_start=1
                     /product="recognition and cleavage site for human 
                     rhinovirus 3C and PreScission proteases"
                     /label=HRV 3C site
                     /translation="LEVLFQGP"
     primer_bind     complement(668..689)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 gggaccacgc gtacccaaca cacggacaca tgcacgaaca cgcatgcaga gagatcatac
       61 atcatctata gagaagtaca cgtaaaagta agactcgtcg tctgccagct acaaccgaga
      121 aggcgacgtg aaaggaacaa gaaaggagga ggagggcggc cgcttacacc actttgtaca
      181 agaaagttgg caatcggggc aagcagccgg ccacagggtc cggccgggga ggcactggca
      241 caaagttcat agggcagagc agcagcgggc acatcttctc gtgctccttc agtgcctctg
      301 tcaggtgctt cagctcctct gtcagcttcc cgatctctct ccgcagcatg gtgttttctt
      361 gctccaggct ctcatattcc tcatggagct tgtcagcctt ctgggtctgc ttcttccgac
      421 ttctctgagc agcaactcgg tttttttctc tccttcggac cttcctgtca tcatcctcag
      481 ggctctgctg ctgcggctgc ggctgcggct ggttcccggg cgccgcgacg ctcctctgca
      541 ggacgctgcc ggcggccggg agcccttgcg acatggtgcc aacttttttg tacaaacttg
      601 tcggaccctg gaagagcacc tccagcttgt atagctcgtc catgccgaga gtgatcccgg
      661 cggcggtcac gaactccagc aggaccatgt gatcgcgctt ctcgttgggg tctttgctca
      721 gggcggactg ggtgctcagg tagtggttgt cgggcagcag cacggggccg tcgccgatgg
      781 gggtgttctg ctggtagtgg tcggcgagct gcacgctgcc gtcctcgatg ttgtggcgga
      841 tcttgaagtt caccttgatg ccgttcttct gcttgtcggc catgatatag acgttgtggc
      901 tgttgtagtt gtactccagc ttgtgccc
//
